This experimental study set out to investigate the protective effects of L-carnitine and gum Arabic supplementations, either alone or in combination, against High Fat Diet (HFD) obesity-related complications in male Wistar rats. A total of 48 three-month-old rats weighing 160–180g were randomly assigned to six groups. The control group was given standard diet and water, while the other groups (T1– T5) were fed with HFD with 30% animal fat for 60 days to induce hyperlipidemia and T5 maintained on a standard diet. After the lipid elevation was confirmed, cure regimens were applied for 30 days: T2=f 600 mg/kg GA, T3= f 250 mg/kg L- Carnitine, T4=Both agents, and T5=Both agents were administered alongside a standard diet.The rats of the untreated HFD group (T1) showed statistically significant higher body weight, serum lipids (total cholesterol (TC), TGs, LDL, VLDL), liver and renal function tests (AST, ALT, urea, creatinine) and atherogenic index, with reduction in HDL, in comparison with that in the control group. On the other hand, the treatment groups, especially T4 and T5 showed obvious ameliorations of lipid metabolism, organ function and body weight regulating. These findings indicate promising role for L-carnitine and gum Arabic against the metabolic disorders associated with obesity. Combined treatment provided better results, which suggested a synergic effect in treatment of diet-induced obesity.