Al-Qadisiyah Journal of Veterinary Medicine Sciences
Official Journal of the College of Veterinary Medicine, University of Al-Qadisiyah

Association Between Glucose, Cholesterol and Total protein of Ovarian Follicular Fluid and Follicle Size in Different Seasons of Iraqi Sheep

Document Type : Research Paper

Authors

Dhia Hussain Jassim 1
Hussam Karim Nassir 2

1 University of Al-Qadissiyah ,Vetetinary Medicine College

2 College of Veterinary Medicine, University of Al-Qadisiyah

https://doi.org/10.29079/qjvms.2025.163318.1105
Abstract
The present study  was undertaken at Al-Qadisiyah slaughter house located in Al-Diwaniyah City, throughout the period from (June –August  2023) and ( Dec. – Feb. 2024) to evaluate certain biochemical components(Glucose. Cholesterol and Total Protein) in the follicular fluid of Iraqi Sheep. A total of (240)ovaries were collected from (120) adult non-pregnant ewes(72 samples of follicular fluids for each size, 12samples/month), age ranging between (2 to 3) years old in a good health with normal genitalia tracts. The diameter of  ovarian follicles were measured using calipers, the follicles were distributed  into three categories: small (<3mm), medium (3-5mm) and large (6-10 mm), follicular fluid aspirated per follicle size within the same ewe and centrifuged at 4000 rpm for 10 min. the supernatant was collected to use for determine  concentration  of glucose, Cholesterol and total protein in the ovarian follicular fluid.  The results shown  that season of year and size of follicle affected significantly(P<0.01)  on glucose and cholesterol concentration, while total protein  concentration did not influenced by season and follicle size, in addition, there was a highly significant differences(P<0.01) recorded among sized follicles within the seasons. However, the concentrations of glucose, cholesterol and total protein in the follicular fluid were decreased as the follicle sizes increased.

Keywords

Sheep
Follicular fluid
Follicle
Biochemical Components
Crossmark
Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 24, Issue 2
December 2025
Pages 150-156

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