Abstract Sheep pox is a highly contagious infectious disease that primarily affects small ruminants such as sheep. The causative agent is a member of the Poxviridae family, and causes severe skin rashes, fever, diarrhea, deaths in young litters and abortions in pregnant ewes. In recent years, many single and multiple cases of SPV have been reported with failure of control and treatment strategies. This indicates that the SPV virus may undergo genetic changes that reduce host immunity, so it had to be studied to diagnose the gene encoded by the virus and identify its sequences, such as the apoptosis gene.   one hundred and twenty-five scab samples were collected from suspected   in the   Diwaniyah Province throughout September 2023 and January 2024, the samples were subjected to a PCR examination to identify the presence of the anti-apoptosis gene.  Result revealed that the expected amplicons size 515 bp of SPV was detected in forty samples (32%) of skin lesions. Number of base substitutions per site between sequences was shown. The identity score of apoptosis related gene of ten positive local isolates was 100% with Abu Gharib _Iraq vaccines Registered breeds in global sequences. Phylogenetic tree analysis based on the partial apoptosis related gene showed that all samples tested were closely related in sequence alignment with NCBI-BLAST capripoxviruses: sheeppox virus envelope protein apoptosis related gene.  This study provided sequence information of anti-apoptotic gene for several SPV isolates, whichpositively affects the epidemiological study of Capripoxvirus