Document Type : Research Paper
Authors
Department of Physiology, Biochemistry, and Pharmacology, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah, Iraq
Abstract
The current research was conducted to explore the feasibility of employing ginseng nanoparticles (NPs) to improve sperm mitochondrial functions in vivo. For this purpose, a total of (60) adult male Wister rats were explored, acclimated at the animal house of the College of Veterinary Medicine at Al-Qadisiyah University. They prepared and divided into three equal groups. T1, T2, and control negative received only distilled water daily and no other treatment. T1 was given a daily dose of 500 mg/kg of ginseng extract orally by stomach tube. While T2 received orally a daily dose of 250 mg/kg of ginseng NPs by stomach tube. After a 60-day experimental period, all study animals were put to anesthesia and dissection to collect testis samples that were subjected to gene expression (real-time RT, polymerase chain reaction PCR) analysis through targeting Succinate dehydrogenase complex flavoprotein subunit A (SDHA) genes. The results of calculating the gene expression of the gene SDHA showed a significant (P<0.05) increase in the level of SDHA gene expression in both the group of rats treated with the Ginseng extract (13.03±1.49) and the group of rats treated with ginseng nanoparticles (48.46±3.85), compared with the negative control group of rats(1±0C). At the same time, the study results showed a significant (P<0.05) increase in the level of gene expression in the group of rats treated with nanoparticles when compared with the group of rats treated with extract only. Using sperm analysis and a molecular biologic approach, this study conformal the impact of ginseng on spermatogenesis in rat testes.
Keywords
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