Document Type : Research Paper
Authors
1 Department of Public Health, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah, Iraq
2 Department of Public Health, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq
Abstract
The objective of this study was to assess the effects of a low protein content (low CP) level diet supplemented with a kemzyme on the growth-prompting effect, the biochemical effects of kemzyme with cGH gene expression, and other related effects. Three (3) groups with (90) birds in each group with (3) replicates, depending on the concentration of protein that met the nutritional requirements of the broiler nutritional composition of the starter, grower, and finisher, were used in our experiment with 270 broiler chicks strain (Ross 308) one day old birds to determine the effect of feeding programs with different CP levels (CP-3%) lower dietary CP content, using at a dose of 500-1000 gm/ton (con). Kemzyme enhanced weight of body and FCR, according to results (p<0.05). levels of serum total protein and albumin were significantly higher in the low CP and kemzyme-fed groups compared to the low protein level-only groups (P<0.05), while the liver and kidney enzymes were unaffected. Kemzyme supplementation slightly significant increased the expression of the cGH gene.
Keywords
1. Bughdadi FA, Attia KA, Sohair YS. A comparative study on the effects of enzymes feed additive "Kemzyme" and antibiotic growth promoter "Salinomycin" on haemostatic mechanism of rabbit bucks. J Agric Sci. 2014;6(1).https://doi.org/10.5539/jas.v6n1p161
2. Butaye LA, Devries LA, Freddy H. Antimicrobial growth promoters used in animal feed: Effects of less well-known antibiotics on gram-positive bacteria. Laboratory of Veterinary Bacteriology and Mycology, Department of Pathology, Faculty of Veterinary Medicine, University of Ghent, Belgium. 2003;16(2):175-88.https://doi.org/10.1128/CMR.16.2.175-188.2003
3. Al-Oubaidi EGA, Shalash AA. Isolation and identification of Lactobacillus salivarus to be used as probiotic in chickens. The Iraqi J Vet Med. 2003;27(1):158-173.https://doi.org/10.30539/ijvm.v27i1.1106
4. Al-Kaissy GA. A comparative between enzyme and yeast chromium in the feed on the intestinal microflora balance in broiler chicks. The Iraqi J Vet Med. 2007;31(1):104-112.https://doi.org/10.30539/iraqijvm.v31i1.809
5. Al-Shadeed MJ. Effect of vinegar added to drinking water on the broiler performance during summer season: Mohammad JB Al-Shadeed; Shahrazad M Al-Shadeed; Najim I Al-Hadeethi; Ammar Q Al-Awssi. The Iraqi J Vet Med. 2010;34(1):9-15.https://doi.org/10.30539/iraqijvm.v34i1.654
6. Al-Hamiari MA. Study the effect of feed withdrawal and adding dried bakery yeast in the diet on pH and some microbial types of broiler chickens intestine. The Iraqi J Vet Med. 2007;31(2):39-50.https://doi.org/10.30539/iraqijvm.v31i2.786
7. Abd Al-Jaleel RA. Use of turmeric (Curcuma longa) on the performance and some physiological traits on the broiler diets. The Iraqi J Vet Med. 2012;36(1):51-57.https://doi.org/10.30539/iraqijvm.v36i1.548
8. Zanella I, Sakomura NK, Silversides FG, Figueirdo A, Pack M. Effect of enzyme supplementation of broiler diets based on maize and soybeans. Poult Sci. 1999;78:561-768.https://doi.org/10.1093/ps/78.4.561
9. Hussein EOS, Suliman GM, Abudabos AM, Alowaimer AN, Ahmed SH, Abd El-Hack ME, Laudadio V. Effect of a low-energy and enzyme-supplemented diet on broiler chicken growth, carcass traits and meat quality. Arch Anim Breed. 2019;62(1):297-304.https://doi.org/10.5194/aab-62-297-2019
10. Sigolo S, Zohrabi Z, Gallo A, Seidavi A, Prandini A. Effect of a low crude protein diet supplemented with different levels of threonine on growth performance, carcass traits, blood parameters, and immune responses of growing broilers. Poultry Science. 2017;96:2751-2760.https://doi.org/10.3382/ps/pex086
11. Fisher T. Specialty poultry production: Impact of genotype, feed strategies, alternative feedstuffs, and dietary enzymes on the growth performance and carcass characteristics of heritage breed chickens. 2016.
12. Centraal Veevoederbureau (C.V.B.). Livestock Feed Table; Tables Values Poultry Nutrition. Lelystad, the Netherlands. 2012.
13. Wang C, Zhao F, Li Z, Jin X, Chen X, Geng Z, Hu H, Zhang C. Effects of resveratrol on growth performance, intestinal development, and antioxidant status of broilers under heat stress. Animals. 2021;11:1427.https://doi.org/10.3390/ani11051427
14. Clinical and Laboratory Standards Institute. EP5-A2 Evaluation of Precision Performance of Quantitative Measurement Methods; Approved Guideline-Second Edition. 2004.
15. Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative CT method. Nat Protocols. 2008;3(6):1101-1108.https://doi.org/10.1038/nprot.2008.73
16. SAS Institute Inc. SAS/STAT 9.13 User's Guide. SAS Inst. Inc., Cary, NC. 2004.
17. Odetallah NH, Wang JJ, Garlich J, Shih JCH. Keratinase in starter diets improves growth of broiler chicks. Poult Sci. 2003;82:664-670.https://doi.org/10.1093/ps/82.4.664
18. Kocher A, Choct M, Ross G, Broz J, Chung TK. Effects of enzyme combinations on apparent metabolisable energy of corn-soybean meal-based diets in broilers. J Applied Poult Res. 2003;12:275-283.https://doi.org/10.1093/japr/12.3.275
19. El-Katcha MI, Soltan MA, El-Kaney HF, Karwarie ER. Growth performance, blood parameters, immune response and carcass traits of broiler chicks fed on graded levels of wheat instead of corn without or with enzyme supplementation. Alexandria J of Vet Sci. 2014;40:95-111.https://doi.org/10.5455/ajvs.48232
20. Attia YA, Al-Khalaifah H, Abd El-Hamid HS, Al-Harthi MA, El-shafey AA. Effect of different levels of multienzymes on immune response, blood hematology and biochemistry, antioxidants status and organs histology of broiler chicks fed standard and low-density diets. Frontier in Vet Sci. 2020;6:510.https://doi.org/10.3389/fvets.2019.00510
21. Choct M. Enzymes for the feed industry: past, present and future. Worlds Poult Sci J. 2006;62:5-16.https://doi.org/10.1079/WPS200480
22. El-Kelawy MI. Effect of feed form, pellet diameter and enzymes supplementation on productive and physiological performance of broiler chicks (Ph.D. thesis), Faculty of Agriculture Damanhour University, Damanhur, Egypt. 2012.
23. Attia YA, El-Tahawy WS, Abd El-Hamid AE, Hassan SS, Nizza A, El-Kelaway MI. Effect of phytase with or without multienzyme supplementation on performance and nutrient digestibility of young broiler chicks fed mash or crumble diets. Ital J Anim Sci. 2012;11:e56.https://doi.org/10.4081/ijas.2012.e56
24. Attia YA. Value of rice bran, its maximal utilization, and upgrading by phytase and other enzymes and diet-formulation based on available amino acid for broiler chicks. Archiv Geflugelk. 2003;67:157-66.
25. Shirmohammad F, Mehr M. Effects of dietary supplementation of multienzyme complex on the energy utilization in rooster and performance of broiler chicks. Afr J Biotechnol. 2011;10:7541-7.
26. Anuradha P, Roy B. Effect of supplementation of fiber degrading enzymes on performance of broiler chickens fed diets containing de-oiled rice bran. Asian Journal of Animal and Veterinary Advances. 2015;10(4):179-184.https://doi.org/10.3923/ajava.2015.179.184
27. Dongare NA, Deshmukh AD, Dhok AP, Lend SR, Taksande PE. Supplementation of protease and xylanase enzymes in broiler diet with varying energy and protein levels. Int J Curr Microbiol App Sci. 2017;6(11):1715-1720.https://doi.org/10.20546/ijcmas.2017.611.207
28. Law FL, Zulkifli I, Soleimani AF, Liang JB, Awad EA. The effects of low-protein diets and protease supplementation on broiler chickens in a hot and humid tropical environment. Asian-Australas J Anim Sci. 2018;31:1291-1300.https://doi.org/10.5713/ajas.17.0581
29. Schmidt EMS, Locatelli-Dittrich R, Santin E, Paulillo AC. Patologia clínica em aves de produção-uma ferramenta para monitorar a sanidade avícola-revisão. Farriers Mag. 2007;1:9-20.https://doi.org/10.5380/avs.v12i3.10906
30. McMurtry JP, Francis GL, Upton Z. Insulin-like growth factors in poultry. Domestic Animal Endocrinology. 1997;14:199-229.https://doi.org/10.1016/S0739-7240(97)00019-2
31. Abraham E, Kight K, Gothilf Y, Du S, Zohar Y. Abstracts of the 15th ICCE. 2005;4:24.
32. Straus DS, Takemoto CD. Specific decrease in liver insulin-like growth factor-I and brain insulin-like growth factor-II gene expression in energy-restricted rats. Journal of Nutrition. 1991;121:1279-1286.https://doi.org/10.1093/jn/121.8.1279
33. Kita K, Nagao K, Taneda N, Inagaki Y, Hirano K, Shibata T, Yaman MA, Conlon MA, Okumura J. Insulin-like growth factor binding protein-2 gene expression can be regulated by diet manipulation in several tissues of young chickens. Journal of Nutrition. 2002;132:145-151.https://doi.org/10.1093/jn/132.2.145
34. Al-Kelabi TJK, Mohamed MF, Rezaeian M, Al-Karagoly H. Growth hormone and growth hormone receptor genes expression related with productive traits of broilers under the effectiveness of the sweet basil plant additive as a growth promoter. Adv Anim Vet Sci. 2019;7(5):361-369.https://doi.org/10.17582/journal.aavs/2019/7.5.361.369
)
