Al-Qadisiyah Journal of Veterinary Medicine Sciences

In Silico Design and Analysis approach, Multi-Epitope Antigen Against Coccidiosis in Broiler Chickens

Document Type : Research Paper

Authors

haider hamza atshan 1
Noor Idan Jarad 2

1 Veterinary Medicine College /Al-Qadisiyah University, Iraq

2 College of Veterinary Medicine, University of Al-Qadisiyah, Iraq,

https://doi.org/10.29079/qjvms.2025.160830.1092
Abstract
Background: The spread of coccidiosis in poultry has been accompanied by challenges in control and treatment, such as resistance to repeated treatments used in the field, and limited immunity due to antigenic diversity, which prompted researchers to adopt the concept of immunogenic antigens. Objectives: To generate a vaccine using computational methods to create broader immunity against mixed infections and longer-lasting immunity to control them. Methods: Using bioinformatics design techniques to provide the highest and most comprehensive level of protection against coccidiosis, the current project introduced the 19 kDa sporozoite antigen as an immunogenic protein genetically conserved among Eimeria species to design a multi-antigen vaccine. Bioinformatics tools were used to generate a vaccine antigen with a total of 18 antigens (B cells, cytotoxic T cells, and high-density T cells) consisting of 274 amino acids. Outcome: The reconstituted vaccine was physically and chemically evaluated, and binding to TLR15 and TLR4 was achieved. Dynamic immunological simulations were performed, using results from immunological compounds and molecules, including antigen levels of 700,000/ml and 500,000/ml, INFy about 4,75,000 ng/ml, and enhanced T and B cell proliferation to produce IgG (30,000/ml). Conclusion: The results of the vaccine design and evaluation simulations were acceptable expectations, and further in vivo experiments are needed to confirm the vaccine's defensive efficacy

Keywords

Eimeria
immunobioinformatic
antigen
vaccine
epitope
Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 25, Issue 1
May 2026
Pages 203-215