Al-Qadisiyah Journal of Veterinary Medicine Sciences
Official Journal of the College of Veterinary Medicine, University of Al-Qadisiyah

Phylogenetic analysis and molecular identification of Staphylococcus species isolated from cheese

Document Type : Research Paper

Authors

Qasim Zamel Bneed
Orooba Meteab Faja

Department of Public Health, College of Veterinary, University of Al-Qadisiyah, Al-Diwaniyah City, Iraq

https://doi.org/10.29079/qjvms.2024.185130
Abstract
The purpose of the study was to identify and characterize the microbes found in the cheese samples that were collected from various Al-Diwaniyah City markets using molecular methods. In Al-Diwaniyah City, 75 cheese samples were collected from three different sources. The samples were cultured on the culture media. Detection of the isolated bacteria were done by using the staining, the culture, and the biochemical examination such as catalase tests, as well as PCR. 75 cheese samples tested positive for S. aureus 37(48%), S. epidermidis 20 (26.6%) and S. intermedius 18(24%). A 16S rRNA gene was used to amplify twenty isolates. All isolates were identified by PCR by using 16S rRNA gene and DNA sequencing. All of the examined isolates showed similarity of (93-99) % to those in the Gen Bank of NCBI.

Keywords

cheese
Staphylococcus Spp
gene 16s rRNA
Phylogenetic analysis
Crossmark
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Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 23, Issue 1
May 2024
Pages 35-40

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