Al-Qadisiyah Journal of Veterinary Medicine Sciences

Influence of PMSG on in vitro oocytes maturation of Iraqi she- Camel

Document Type : Research Paper

Authors

Department of Gynecology and Surgery, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq.

10.29079/qjvms.2020.179346

Abstract

The aim of the present study was know the role of PMSG in invitro maturation of she-camel oocytes. Ovaries where collected from Afak slaughterhouse and transport by cool box contain normal saline 0.9% (20-25C°) supplement with (penicillin G sodium 10^4 IU, streptomycin sulfate10mg and Amphotericin B 0.025 mg) to laboratory of Al-Diwaniyah veterinary Hospital during 1-2 hours. Ovaries with visible follicles aspirated by 22-gauge needle attached to 10ml syringe and slicing the ovaries after aspiration. Only type A and B selected and matured in maturation medium (M199-A) supplied with 0, 2, 4, 10 IU of PMSG and incubated in CO2 incubator at 5% CO2, 38.5 Cº, and 90% humidity for 24h. The results was Maturation media supplement with 0IU of PMSG gave lower rate 24% of expended of cumulus cell and 0% appearance of the first polar body (F.P.B) than other groups. However, 10 IU of PMSG gave higher maturation rate 65.3% cumulus expended and 64.7% appearance of the first polar body (F.P.B).

 

Keywords

References
1. Skidmore J, editor. Developments in reproduction in dromedary camels. Satellite meeting on camelid reproduction; 2008.
2. El-Bahray KA. Old World Camel reproduction: nature, current technologies and future prospects. The camel Conferences, SOAS is open to members of the general public. 2011. p. 61-73.
3. Pandey A, Gupta SC, N G. Effect of FSH and LH hormones on oocyte maturation of buffalo and gene expression analysis of their receptors and Cx43 in maturing oocytes. Zygote. 2010;18:231-4.
   [https://doi.org/10.1017/S096719940999030X](https://doi.org/10.1017/S096719940999030X)
4. Eppig J. FSH stimulates hyaluronic acid synthesis by oocyte-cumulus cell complexes from mouse preovulatory follicles. Nature. 1979;281:483-4.
   [https://doi.org/10.1038/281483a0](https://doi.org/10.1038/281483a0)
5. Abd El-Aziz A, Mahrous UE, Kamel SZ, AA S. Factors influencing in vitro production of bovine embryos: a review. Asian J Anim Vet Adv. 2016;11:737-56.
   [https://doi.org/10.3923/ajava.2016.737.756](https://doi.org/10.3923/ajava.2016.737.756)
6. Keefer C, SL S, M M-L. Bovine embryo development in vitro: effect of in vitro maturation conditions on fertilization and blastocyst development. Theriogenology. 1991;35:223.
   [https://doi.org/10.1016/0093-691X(91)90199-N](https://doi.org/10.1016/0093-691X(91)90199-N)
7. Abdoon A, Kandil OM, Otoi T, T S. Influence of oocyte quality, culture media and gonadotropins on cleavage rate and development of in vitro fertilized buffalo embryos. Anim Reprod Sci. 2001;65:215-23.
   [https://doi.org/10.1016/S0378-4320(01)00079-3](https://doi.org/10.1016/S0378-4320(01)00079-3)
8. Abdelkhalek A, Gabr SA, Khalil W, Shamiah S, Pan L, Qin G, et al. In vitro production of Sudanese camel (Camelus dromedarius) embryos from epididymal spermatozoa and follicular oocytes of slaughtered animals. Polish J Vet Sci. 2017;20(1):95-101.
   [https://doi.org/10.1515/pjvs-2017-0013](https://doi.org/10.1515/pjvs-2017-0013)
9. Mayada AE, Nadia A. Taha, Hammam A-MM, Sawiress FAR. Effect of antioxidant supplementation on in vitro maturation of Camelus dromedarius oocytes. Nat Sci. 2015;13(2):17-24.
10. Schroeder A, Schultz R, Kopf G, Taylor F, Becker R, Eppig J. Fetuin inhibits zona pellucida hardening and conversion of ZP2 to ZP2f during spontaneous mouse oocyte maturation in vitro in the absence of serum. Biol Reprod. 1990;43:891-7.
    [https://doi.org/10.1095/biolreprod43.5.891](https://doi.org/10.1095/biolreprod43.5.891)
11. Wei S, Gong Z, Zhao H, Liang H, Lai L, Deng YJ. Equine chorionic gonadotropin influence on sheep oocyte in vitro maturation, apoptosis, and follicle-stimulating hormone receptor and luteinizing hormone receptor expression. Genet Mol Res. 2016;15(4):1-12.
    [https://doi.org/10.4238/gmr15049162](https://doi.org/10.4238/gmr15049162)
12. Gupta P, Nandi S, Ravindranatha B, Sarma PJ. Effect of buffalo follicular fluid alone and in combination with PMSG and M199 on in vitro buffalo oocyte maturation. Asian-Australas J Anim Sci. 2001;14(5):693-6.
    [https://doi.org/10.5713/ajas.2001.693](https://doi.org/10.5713/ajas.2001.693)
 
Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 19, Issue 2
June 2020
Pages 27-31
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