Al-Qadisiyah Journal of Veterinary Medicine Sciences

Efficacy of Silicone Gel Plus Thrombin Adhesives on Cutaneous 2nd Degree Burn Healing

Document Type : Research Paper

Authors

1 Department of Surgery and Obstetric, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq.

2 Department of Surgery and Obstetric/College of Veterinary Medicine/ University of Al-Qadisiyah, Iraq.

Abstract

A lot of researchers try many types of therapies for burns healing in Veterinary medicine in the last decades. Silicone gel may decrease the scar tissue, while thrombin may increase the proliferation of cells. This project aims to compare the efficacy of silicone gel combined and thrombin on 2nd degree cutaneous burn healing. Forty female white albino rats, who were housed in plastic boxes divided randomly into four groups 10 rats for each. Control group G1 had no treatment, second group G2 treated with silicone gel, third group treated with autologous thrombin adhesive, fourth group treated with combination of silicone gel plus autologous thrombin adhesive. Under routine surgery with general anesthesia all rats had thermal injuries at their backs. The thermal injuries were then created using a solid aluminum bar with a diameter of 2 cm in diameter had previously been heated to 100°C in boiling water. The bar is kept in touch with the rat's skin in the dorsal proximal region of the back. Hyperemia, edema, scar tissue, and scab were recorded for morphological exam. The therapies were applied topically once daily for 10 days. At the time of burn and on the appropriate days for the biopsies at 7th ,14th,21th, and 28th day, sterile "swabs" were taken and sent to laboratory for bacterial contamination. Biopsies were collected for histopathologic evaluation of healing. The morphologic appearance was recorded at 1 day, 7th day, 14th day,21th day, and 28th day, it was the best in G3. A completed wound healing was recorded highly significant wound contraction in G3 groups. The bacterial colonies count was recorded the lowest in G3. Histopathologic evaluation showed the superiority of thrombin adhesive than silicone gel for 2nd degree burns healing and this result accompanied with the other parameters. Histological changes post burn injury in thrombin treatment showed complete healing of all skin layers including epidermis, papillary dermis, reticular dermis, and hypodermis, hair follicles, sweat glands, and sebaceous glands were obvious while in silicone gel showed regenerated epidermis layer, gaps between epidermis and dermis, edema or bulla like lesion between epidermis and dermis, subcutaneous edema, and infiltration of inflammatory cell in the dermis mostly lymphocytes.E. The study concluded that silicone gel decreases scar formation and thrombin accelerate burn healing.
 

Keywords

References
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Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 23, Issue 2
December 2024
Pages 18-28
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