Al-Qadisiyah Journal of Veterinary Medicine Sciences
Official Journal of the College of Veterinary Medicine, University of Al-Qadisiyah

Investigation the effects of green synthesized ZnO nanoparticles on the viability of L929 fibroblasts using MTT assay

Document Type : Research Paper

Authors

Asseel Abdulridha Saeed 1
Mushtaq Talib Abdulwahid 2

1 College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah City, Iraq

2 College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq

https://doi.org/10.29079/qjvms.2023.183465
Abstract
Zinc oxide nanoparticles (ZnONPs) were prepared by eco-friendly method   using the alcoholic extract of Ginger(  Zingiber officinale) ,   this study conducted to evaluate the cytotoxic effect of  Zinc oxide nanoparticles on  viability of    L929  fibroblast cell line  for 72 hours   and  incubation with (1, 2, 4, 8, and 16 mg/ml) of GnZnO NPs , Several methods were used to characterize the biosynthesized GnZnONPs. including "UV-Visible Spectroscopy," Field Emission Scanning Electron Microscope ("FESEM"), " Infrared Spectroscopy (FTIR)" and X-Ray Diffraction (XRD) spectrum.  the MTT assay was used  to determine the   cytotoxicity  effect  of Zinc oxide nanoparticles (ZnONPs)  on  the,  cell line  viability The results showed that there was no toxic effect of the synthesis GnZnONPs with an increase in concentration from (1-8) mg/ml after 72 hours of incubation at 37°C.  The findings of this study, there was a time and dose-dependent reduction in cell viability in treated cell lines after exposure to GnZnO-NPs for 3 days, with no adverse effect on fibroblast cells till 8 mg/ml.

Keywords

ZnONPs
Zingiber officinale
MTT assay
L929 fibroblast
Crossmark
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