Al-Qadisiyah Journal of Veterinary Medicine Sciences
Official Journal of the College of Veterinary Medicine, University of Al-Qadisiyah

Effect of Nano-Piperine and Vitamin C in Antioxidant Status in Dexamethasone Treated Male Rats

Document Type : Research Paper

Authors

Zainab Sa'ad Al_Hassani
Sa'adeya Ali Al-Gnami

Department of Physiology and Pharmacology, College of Veterinary Medicine, University of AL-Qadisiyah, Iraq

https://doi.org/10.29079/qjvms.2023.183455
Abstract
The study was designed to investigate whether nano-piprine and vitamin C (Vit. C) have antioxidant activity and/or it act through induction of the endogenous antioxidants. The study involved 48 male Wister rats (90 days old, 150±10g weight) randomly assigned to 6 groups (8 per group) and administered orally for 30 days. T1 was given 15 mg/kg B.W. nano-piperine, T2 was given 25 mg/kg B.W. vitamin C, T3 was given 2mg/kg B.W. dexamethasone, T4 was given both at the same time, and T5 was given both at the same time. Malondialdehyde (MDA) concentration and activities of superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH).  were measured in blood serum. The study found a substantial rise (p<0.05) in MDA levels in the T3 group given dexamethasone compared to the control group. Significant reduction (p<0.05) in MDA levels was seen in T4 and T5 groups compared to T3. Additionally, MDA levels increased significantly (p<0.05) in T1 and reduced in T2 compared to the control group. The T1 and T2 groups showed a substantial increase (p<0.05) in SOD, CAT, and GSH compared to the control group. T3 had the lowest SOD, CAT, and GSH and other experimental groups. Additionally, T4 and T5 showed a substantial increase (p<0.05) in these parameters compared to T3. It can be concluded that nano-piprine and Vit. C has antioxidant activity and plays a great role in reducing free radicals and increasing endogenous antioxidants.  

Keywords

Dexamethasone
Antioxidants
Nano-Piperine
Vit.C
Crossmark
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