Document Type : Research Paper
Authors
Department of Physiology and Pharmacology, College of Veterinary Medicine, University of AL-Qadisiyah, Iraq
Abstract
The study was designed to investigate whether nano-piprine and vitamin C (Vit. C) have antioxidant activity and/or it act through induction of the endogenous antioxidants. The study involved 48 male Wister rats (90 days old, 150±10g weight) randomly assigned to 6 groups (8 per group) and administered orally for 30 days. T1 was given 15 mg/kg B.W. nano-piperine, T2 was given 25 mg/kg B.W. vitamin C, T3 was given 2mg/kg B.W. dexamethasone, T4 was given both at the same time, and T5 was given both at the same time. Malondialdehyde (MDA) concentration and activities of superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH). were measured in blood serum. The study found a substantial rise (p<0.05) in MDA levels in the T3 group given dexamethasone compared to the control group. Significant reduction (p<0.05) in MDA levels was seen in T4 and T5 groups compared to T3. Additionally, MDA levels increased significantly (p<0.05) in T1 and reduced in T2 compared to the control group. The T1 and T2 groups showed a substantial increase (p<0.05) in SOD, CAT, and GSH compared to the control group. T3 had the lowest SOD, CAT, and GSH and other experimental groups. Additionally, T4 and T5 showed a substantial increase (p<0.05) in these parameters compared to T3. It can be concluded that nano-piprine and Vit. C has antioxidant activity and plays a great role in reducing free radicals and increasing endogenous antioxidants.
Keywords
- Fang YZ, Yang S, Wu G. Free radicals, antioxidants, and nutrition. Nutrition. 2002;18:872-879.https://doi.org/10.1016/S0899-9007(02)00916-4
- Poljsak B, Milisav I. The Neglected Significance of "Antioxidative Stress". Oxid Med Cell Longev. 2012;2012:480895.https://doi.org/10.1155/2012/480895
- Chovanová K, Böhmer M, Poljovka A, Budiš J, Harichová J, Szemeš T, Zámocký M. Parallel Molecular Evolution of Catalases and Superoxide Dismutases-Focus on Thermophilic Fungal Genomes. Antioxidants. 2020;9:1047.https://doi.org/10.3390/antiox9111047
- Vijayakumar R, et al. Antioxidant efficacy of black pepper (Piper nigrum L.) and piperine in rats with high-fat-diet-induced oxidative stress. Redox Report. 2004;9:105-108.https://doi.org/10.1179/135100004225004742
- Ahmed AE, Alshehri A, Al-Kahtani MA, Elbehairi SEI, Alshehri MA, Shati AA, Alfaifi MY, Al-Doais AA, Taha R, Morsy K, El-Mansi AA. Vitamin E and selenium administration synergistically mitigates ivermectin and doramectin-induced testicular dysfunction in male Wistar albino rats. Biomed Pharmacother. 2020;124:109841.https://doi.org/10.1016/j.biopha.2020.109841
- Meghwal M, Goswami T. Piper nigrum and piperine: An update. Phytother Res. 2013;27(8):1121-1130.https://doi.org/10.1002/ptr.4972
- Patel S, et al. Influence of co-administration of piperine on pharmacokinetic profile of gatifloxacin in layer birds. Global Veterinaria. 2011;7:427-32.
- Ismail OO, Ridwan IB, Olaiya OG, Abdulbasit A, Rukayat JO, Roehan FO, Abass SA. Effects of ascorbic acid on immunosuppressive drug-azathioprine-induced alteration in the liver and kidney of adult waster rats [Rattus norvegicus]. Eur J Exp Biol. 2013;3(2):371-378.
- Razzaq SA, Jaber IJ, Kadhim SA, Abbas AA. Pharmacological effects of dexamethasone in rats. Indian J Forensic Med Toxicol. 2020;14(3).
- Joda M. The Progressive Statistical Analysis by Using SPSS. First Ed. Wals House Editions, Amman Jordan; 2008.
- Nguelefack-Mbuyo EP, Peyembouo FP, Fofié CK, Nguelefack TB. Dose-dependent and time-dependent metabolic, hemodynamic, and redox disturbances in dexamethasone-treated Wistar rats. J Basic Clin Physiol Pharmacol. 2021.https://doi.org/10.1515/jbcpp-2020-0365
- Mahmoud HS, Almallah AA, Gad EL-Hak HN, Aldayel TS, Abdelrazek HMA, Khaled HE. The effect of dietary supplementation with Nigella sativa (black seeds) mediates immunological function in male Wistar rats. Sci Rep. 2021;11:7542.https://doi.org/10.1038/s41598-021-86721-1
- Alli J, Kehinde A, Kosoko A, Ademowo O, Adeolu A. Oxidative Stress and Reduced Vitamins C and E Levels Are Associated with Multi-Drug Resistant Tuberculosis. J Tuberc Res. 2014;2:52-58.https://doi.org/10.4236/jtr.2014.21006
- Adeyemi OS, Afolabi LB, Rotimi DE, Atanu FO, Ofume DS, Ogunleye T, Oluwalana DO, Oluwole TP, Youssef A, Mostafa-Hedeab G, Batiha GE-S. Differential Immunomodulatory Potential of Silver Nanoparticles and Effect on the Kynurenine Pathway in Male Wistar Rats. J Nanomater. 2022;2022:e9883142.https://doi.org/10.1155/2022/9883142
- Salihu M, Batiha GE-S, Kasozi KI, Zouganelis GD, Sharkawi SMZ, Ahmed EI, Usman IM, Nalugo H, Ochieng JJ, Ssengendo I, Okeniran OS, Pius T, Kimanje KR, Kegoye ES, Kenganzi R, Ssempijja F. Crinum jagus (J. Thomps. Dandy): Antioxidant and protective properties as a medicinal plant on toluene-induced oxidative stress damages in liver and kidney of rats. Toxicol Rep. 2022;9:699-712.https://doi.org/10.1016/j.toxrep.2022.03.026
- Mrityunjaya M, Pavithra V, Neelam R, Janhavi P, Halami PM, Ravindra PV. Immune-Boosting, Antioxidant and Anti-inflammatory Food Supplements Targeting Pathogenesis of COVID-19. Front Immunol. 2020;11:570122.https://doi.org/10.3389/fimmu.2020.570122
- Reda FM, Alagawany M, Mahmoud HK, Mahgoub SA, Elnesr SS. Use of red pepper oil in quail diets and its effect on performance, carcass measurements, intestinal microbiota, antioxidant indices, immunity and blood constituents. Animal. 2020;14(5):1025-1033.https://doi.org/10.1017/S1751731119002891
- Safaeian L, Zabolian H. Antioxidant Effects of Bovine Lactoferrin on Dexamethasone-Induced Hypertension in Rat. ISRN Pharmacol. 2014;2014:943523.https://doi.org/10.1155/2014/943523
- Mobarez E, Azoz H, Alkalamawy N, Nada A-F. Evaluation the Effectiveness of Green Zinc Oxide Nanoparticles on The Anti-Inflammatory Effect of Dexamethasone and Its Side Effects in Rats. SVU-Int J Vet Sci. 2018;1:25-54.https://doi.org/10.21608/svu.2018.17924
- Brusini R, Varna M, Couvreur P. Advanced nanomedicines for the treatment of inflammatory diseases. Adv Drug Deliv Rev. 2020;157:161-178.https://doi.org/10.1016/j.addr.2020.07.010
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