Al-Qadisiyah Journal of Veterinary Medicine Sciences

Human Recombinant Interleukin-3 (hrIL-3) Stimulates FSHR and LHCGR Expression in Ovaries of Female Rats

Document Type : Research Paper

Authors

Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, University of Al-Qadisiyiah, Al-Diwaniyah, Iraq

Abstract

   To investigate the impact of human recombinant interleukin-3 (hrIL-3) on ovulation in female rats ovaries. We used forty eight Wister  rats were divided randomly into four equal groups twelve female each. All rats undergone superovulation protocols using pregnant mare serum gonadotropin (PMSG) 10IU intra-peritoneally. Final maturation of oocytes achieved after 48hours using human chorionic gonadotropin (hCG) 10IU intra-peritoneally. We used hrIL-3 in three doses: 15ng, 30ng, and 45ng/rat after superovulation. Concomitantly with hCG, intravenous injection of hrIL-3 was used in three doses o the three treated groups,  while the control group received normal saline at the same time of hCG injection. The animals of each group were divided into two subgroups  six female each According to the time of sacrificing after 12h and 36h of hCG injection. Samples of ovaries were obtainedv from all animals for RT-qPCR gene expression of  fshr and lhcgr.   There was a  significant increase (P<0.05) of fshr gene after 12h of treatment in 15ng and 45ng hrIL-3 compared with control. However, there were no significant differences among the other groups  compared to control. There is significant increase (P<0.05) of lhcgr gene after 12h of treatment in 15ng hrIL-3 compared with control, while there were no significant differences among the other groups. After 36h, lhcgr gene showed significant increase (P<0.05) of 45ng compared with control while there were no significant differences among the other groups.hrIL-3 with a dose of 45ng at 36h after hCG injection stimulates rat ovaries via increase gene expression of  FSHR and LHCGR in ovarian tissues.

Keywords

References
1. Gershon E, Dekel N. Newly Identified Regulators of Ovarian Folliculogenesis and Ovulation. Int J Mol Sci. 2020;21:4565. https://doi.org/10.3390/ijms21124565
2. Kishi H, Kitahara Y, Imai F, Nakao K, Suwa H. Expression of the gonadotropin receptors during follicular development. Reprod Med Biol. 2018;17:11-9. https://doi.org/10.1002/rmb2.12075
3. McGee EA, Hsueh AJ. Initial and cyclic recruitment of ovarian follicles. Endocr Rev. 2000;21(2):200-14. https://doi.org/10.1210/edrv.21.2.0394
4. Hillier SG. Gonadotropic control of ovarian follicular growth and development. Mol Cell Endocrinol. 2001;179:39-46. https://doi.org/10.1016/S0303-7207(01)00469-5
5. Hennet ML, Combelles CM. The antral follicle: a microenvironment for oocyte differentiation. Int J Dev Biol. 2012;56:819-30. https://doi.org/10.1387/ijdb.120133cc
6. Orsi NM, Baskind NE. Cummings M, Wilkinson N, editors. Pathology of the ovary, fallopian tube and peritoneum, essentials of diagnostic gynecological pathology. In: Anatomy, development, histology, and normal function of the ovary. 2014. https://doi.org/10.1007/978-1-4471-2942-4_1
7. Casarini L, Crépieux P. Molecular Mechanisms of Action of FSH. Front Endocrinol. 2019;10:305. https://doi.org/10.3389/fendo.2019.00305
8. Gougeon A. Intragonadal regulation of human follicular genesis: facts and hypotheses. Endocr Rev. 1994;15(2):63-73.
9. Lussier JG, Diouf MN, Levesque V, Sirois J, Ndiaye K. Gene expression profiling of upregulated mRNA in granulosa cells of bovine ovulatory follicles following stimulation with hCG. Reprod Biol Endocrinol. 2007;5:1-16. https://doi.org/10.1186/12958-017-0306-x
10. Loukides JA, Loy RA, Edwards R, Honig J, Visintin I, Polan ML. Human follicular fluid contains tissue macrophages. J Clin Endocrinol Metab. 1990;71:1363-7. https://doi.org/10.1210/jcem-71-5-1363
11. Kokia E, Adashi EY. Potential role of cytokines in ovarian physiology: the case of interleukin-1. In: Adashi EY, Leung PCK, editors. The Ovary. New York: Raven Press; 1993. p. 383-94.https://doi.org/10.1210/edrv-11-3-454
12. Richards JS, Liu Z, Shimada M. Immune-like mechanisms in ovulation. Trends Endocrinol Metab. 2008;19(6):191-6.https://doi.org/10.1016/j.tem.2008.03.001
13. Richards JS, Russell DL, Ochsner S, Espey LL. Ovulation: new dimensions and new regulators of the inflammatory-like response. Annu Rev Physiol. 2002;64:69-92.https://doi.org/10.1146/annurev.physiol.64.081501.131029
14. Yamamoto Y, Kuwahara A, Taniguchi Y, Yamasaki M, Tanaka Y, Mukai Y, et al. Tumor necrosis factor alpha inhibits ovulation and induces granulosa cell death in rat ovaries. Reprod Med Biol. 2015;14:107-15. https://doi.org/10.1007/s12522-014-0201-5
15. Talib AJ, Alhilali MJ. Folliculogenesis and ovulation enhanced by human recombinant hrIL-3 in female rats. Bull Environ Pharmacol Life Sci. 2023;12(5):215-25.
16. Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative CT method. Nat Protoc. 2008;3:1101-8. https://doi.org/10.1038/nprot.2008.73
17. Xie Y, Xiao L, Li S. Effects of Metformin on Reproductive, Endocrine, and Metabolic Characteristics of Female Offspring in a Rat Model of Letrozole-Induced Polycystic Ovarian Syndrome With Insulin Resistance. Front Endocrinol (Lausanne). 2021;12:1-12. https://doi.org/10.3389/fendo.2021.701590
18. Conn PPM, Crowley WF. Gonadotropin-releasing hormone and its analogs. Annu Rev Med. 1994;45:391-405.https://doi.org/10.1146/annurev.med.45.1.391
19. Brännström M, Enskog A. Leukocyte networks and ovulation. J Reprod Immunol. 2002;57:47-60.https://doi.org/10.1016/S0165-0378(02)00009-8
20. Lee EB, Chakravarthi VP, Wolfe MW, Rumi MAK. ERβ Regulation of Gonadotropin Responses during Folliculogenesis. Int J Mol Sci. 2021;22:10348. https://doi.org/10.3390/ijms221910348
21. Richards JS, Ascoli M. Endocrine, paracrine, and autocrine signaling pathways that regulate ovulation. Trends Endocrinol Metab. 2018;29:313-25. https://doi.org/10.1016/j.tem.2018.02.012
22. Hsueh AJ, Rauch R. Ovarian Kaleidoscope database: ten years and beyond. Biol Reprod. 2012;86:192.https://doi.org/10.1095/biolreprod.112.099127
23. Dierich A, Sairam MR, Monaco L, Fimia GM, Gansmuller A, LeMeur M, et al. Impairing follicle-stimulating hormone (FSH) signaling in vivo: targeted disruption of the FSH receptor leads to aberrant gametogenesis and hormonal imbalance. Proc Natl Acad Sci U S A. 1998;95:13612-7.https://doi.org/10.1073/pnas.95.23.13612
24. Robker RL, Hennebold JD, Russell DL. Coordination of Ovulation and Oocyte Maturation: A Good Egg at the Right Time. Endocrinol. 2018;159(9):3209-18. https://doi.org/10.1210/en.2018-00485
25. Orisaka M, Miyazaki Y, Shirafuji A, Tamamura C, Tsuyoshi H, Tsang BK, Yoshida Y. The role of pituitary gonadotropins and intraovarian regulators in follicle development: A mini-review. Reprod Med Biol. 2021;20:169-75.https://doi.org/10.1002/rmb2.12371
Lim E, Choi Y. Transcription factors in the maintenance and survival of primordial follicles. Clin Exp Reprod Med. 2012;39(4):127-31. https://doi.org/10.5653/cerm.2012.39.4.127
Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 22, Issue 2
September 2023
Pages 64-71
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