Al-Qadisiyah Journal of Veterinary Medicine Sciences

Effect Intra-Testicular Injection of Autologous ‎Platelet-Rich Plasma on Sperm Parameters and Regulation of the CFAP65 and ‎SPEF2 ‎genes‎‏ in Heat-Stressed Rabbits

Document Type : Research Paper

Authors

1 Department of Life Sciences, Faculty of Sciences-Sfax, University of Sfax, Tunisia

2 Laboratory of histology, embryology and biology of reproduction and withdraw cytogenetics, Faculty of Medicine of Sfax, University of Sfax, Tunisia

3 Department of Surgery and Obstetrics, Faculty of veterinary medicine, University of Al-Qadisiyah, Iraq

Abstract

Autologous ‎Platelet-Rich Plasma (PRP) has a potential effect on tissue repair ‎through the proliferation and ‎differentiation of tissue progenitor cells and is considered simple, safe, ‎easy to prepare and ‎use, easily applicable, and effective in use. Twenty New Zealand White rabbit were used and divided into two groups control (n=10), and the PRP group (n=10) which intratesticular injected with PRP (‎200 ‎μl ‎ PRP ‎per testes‎). In summer season, all animals were ‎daily exposed to sunlight in the middle of the day for ten weeks of June, July, and August. The rectal temperature and temperature humidity index were ‎obtained twice every week after exposure. The sperm parameters and gene expression of the target gene were analyzed before treatment and after 10 weeks. The Computer Assisted Semen Analysis (CASA) results showed that the ‎sperm motility parameters were ‎significantly (P<0.05) better ‎for the summer heat-stressed in bucks injected with PRP than the control ‎group. On the other hand, the gene expression of specific genes responsible for sperm ‎motility ‎ (CFAP65 and SPEF2) results ‎were noted that a ‎high significant regulation (p<0.05) in the PRP group compared with the control. In conclusion, the PRP enhances the sperm parameters of rabbits that suffer from heat stress, as well as increases ‎the regulation of ‎CFAP65 and ‎SPEF2 ‎genes‎‏.‏‎

Keywords

References
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Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 22, Issue 1
May 2023
Pages 1-10
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