Document Type : Research Paper
Author
Intermediate school Youssef Al siddiq, Al-Kut City, Iraq.
Abstract
The research focused on evaluating goat milk's ability to improve renal function and antioxidant status in rats with nephrotoxicity through non-invasive biochemical and imaging techniques. Male Wistar rats (n=30) were randomly divided into three groups: control, nephrotoxic, and goat milk-treated. The researchers created nephrotoxicity by administering intraperitoneal injections of gentamicin at 80 mg/kg/day for a period of 10 days. Every day the goat milk-treated group received 10 mL/kg of fresh goat milk by mouth while taking gentamicin. The assessment of renal function involved measuring serum creatinine levels as well as blood urea nitrogen (BUN) together with urinary albumin-to-creatinine ratio (UACR) and glomerular filtration rate (GFR) through FITC-sinistrin clearance. The researchers measured oxidative stress markers which included malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH) in plasma and urine samples. Researchers utilized contrast-enhanced ultrasonography (CEUS) to study kidney function through real-time assessment of renal tissue oxygenation and perfusion without histological analysis. The nephrotoxic group demonstrated higher serum creatinine, BUN, and UACR levels than the goat milk supplemented group which showed significant reductions (p<0.05). Measurements showed enhanced clearance of FITC-sinistrin indicating significant improvement in GFR for the goat milk-treated group. The antioxidant assays showed that the levels of MDA decreased while SOD, CAT, and GSH activities increased in the goat milk treatment group. The CEUS imaging showed better blood flow and oxygen levels in kidneys of rats fed goat milk which matched the biochemical test results. Goat milk treatment successfully reverses gentamicin-induced kidney damage by restoring normal kidney function.
Keywords
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