Al-Qadisiyah Journal of Veterinary Medicine Sciences
Official Journal of the College of Veterinary Medicine, University of Al-Qadisiyah

Molecular detection and phylogenetic of Cryptosporidium spp. of birds in the city of Al-Diwaniyah, Iraq

Document Type : Research Paper

Authors

Niran Abed Al-zubaidi
Alaa Mohammed Abdulrazaq Al Khafaji

College of Veterinary Medicine, University of Al-Qadisiyah, Al-Qadisiyah, Iraq

https://doi.org/10.29079/qjvms.2024.155646.1052
Abstract
Many vertebrate species, including birds, are susceptible to the important protozoan Cryptosporidium. Evaluating the public health risk associated with Cryptosporidium requires a thorough understanding of species diversity and their distribution across populations. Poultry feces samples were obtained from Al-Diwaniyah, Iraq, and the aim of this study was to investigate their molecular identity and phylogenetic patterns for Cryptosporidium species identification, 70 feces samples were collected from birds and molecular identification by Nested PCR Using phylogenetic analysis and sequencing of positive PCR results was used to identify Cryptosporidium spp. The molecular method had a much higher detection rate than microscopic observation, 8 samples (11.42% of total) showed Cryptosporidium oocysts, while 18 samples (25.71% of total) showed positive results from nested PCR in Phylogenetic analysis showed Three Cryptosporidium species: Cryptosporidium baileyi (61.11%), . Cryptosporidium gallii (26.77%), and Cryptosporidium melegridis (11.11%) comparing local Cryptosporidium isolates from the NCBI GenBank database with expression sequences ranging from 97.80% to 99.45%. Up to the genetic homogeneity known Iraq The birds of Al-Diwaniyah have been infected with various Cryptosporidium species, and this study provides the first molecular data on this topic. these results demonstrate the importance of accurate identification of Cryptosporidium species and how this virus can be transmitted from birds to humans in the field using simple genetic methods to date.

Keywords

Birds
Cryptosporidium
nested PCR
phylogenetic analysis
Crossmark
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Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 24, Issue 1
January 2025
Pages 104-109

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