Al-Qadisiyah Journal of Veterinary Medicine Sciences

Investigation of fungal isolates of chronic rhinitis in sheep

Document Type : Research Paper

Authors

Department of Internal and Preventive Veterinary Medicine, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq

10.29079/qjvms.2024.152833.1035

Abstract

Fungal rhinitis is an important infection in sheep characterized by its chronic status. The study was conducted to clinically and in vitro characterize fungal rhinitis in sheep. The current study was conducted to clinically examine chronic suspected fungal rhinitis in sheep and to isolate fungal species from the lesions and characterize these isolates using molecular techniques. Clinical examination and nasal endoscopy were used first to identify clinical status and nasal lesions in 80 sheep distributed over nine livestock farms in Babylon Province, Iraq. Then, a total of 160 lesion nasal swabs (80 for direct smear and 80 for cultivation) were collected from 80 sheep. The swabs were stained with Grocott's methenamine silver stain (GMS) to identify fungal hyphae. Furthermore, polymerase chain reaction (PCR) and partial gene sequencing methods were performed based on the 18S rRNA gene. The results of the clinical examination of each animal revealed slightly increases in the body temperature, emaciation, nasal congestion, nasal mucoid, mucopurulent, or blood-stained discharge, sneezing, nasal itching. Moreover, varied types of nasal lesions were visualized by endoscope. For the cultivation of the swabs, the findings demonstrated the isolation of 55/80(68.75%) isolates. The endoscopy showed various types of chronic lesions, such as nodules and ulcerations. Based on the molecular methods, the results revealed the presence of seven species of fungi; 13(16.25%) Aspergillus terrusre,10(12.5%) A. flavus,11 (13.75) A.niger,9(11.25%)Rhizopus arrhazus,7(8.75%)Alternaria alternate,3(3.75%)A.tenesumma, and 2(2.5%) Penicillumsoppi.The phylogenetic analysis recorded 20 distinct isolates that were similar in their sequence to global isolates from Saudi Arabia, India, Pakistan, and China. The impact of the study can be emphasized that chronic rhinitis in sheep was primarily caused by Aspergillus species. These isolates were sequencing-based similar to those isolated from different world regions indicating high evolution in the current isolates and more need for improvement of animal health and management. However, the study comes with limitation of the area size sampled, which can be solved with future studies.

Keywords

References
  1. Semenova V, Rodrigues Hoffmann A, Wolking RM, Choi EA (2024). Facial and systemic mucormycosis caused by Lichtheimia corymbifera in a goat: case report and literature review of fungal infections in goats. Journal of Veterinary Diagnostic Investigation, 36(3):463-467. https://doi.org/10.1177/10406387241236751
  2. Cao X, Gu L, Gao Z, Fan W, Zhang Q, Sheng J, Zhang Y, Sun Y (2024). Pathogenicity and Genomic Characteristics Analysis of Pasteurella multocida Serotype A Isolated from Argali Hybrid Sheep. Microorganisms, 12(6):1072. https://doi.org/10.3390/microorganisms12061072
  3. Connell JT, Bouras G, Yeo K, et al. (2024). Characterising the allergic fungal rhinosinusitis microenvironment using full-length 16S rRNA gene amplicon sequencing and fungal ITS sequencing. Allergy, 00:1-13. https://doi.org/10.1111/all.16240
  4. Carmo PMS do, Uzal FA, Pedroso PMO, Riet-Correa F (2020). Conidiobolomycosis, cryptococcosis, and aspergillosis in sheep and goats: a review. Journal of Veterinary Diagnostic Investigation, 32(6):826–834. https://doi.org/10.1177/1040638720958338
  5. Sharma P, Rana T (2023). Fungal Diseases of Goats. Principles of Goat Disease and Prevention, 111–126. https://doi.org/10.1002/9781119896142.ch9
  6. Cordeiro Rde A, Bittencourt PV, Brilhante RS, Teixeira CE, Castelo-Branco Dde S, Silva ST, De Alencar LP, Souza ER, Bandeira Tde J, Monteiro AJ, Sidrim JJ, Rocha MF. Species of Candida as a component of the nasal microbiota of healthy horses. Med Mycol. 2013 Oct;51(7):731-6. doi: 10.3109/13693786.2013.777858. Epub 2013 May 8. PMID: 23651178.
  7. Botero LE, Delgado-Serrano L, Cepeda ML, Bustos JR, Anzola JM, Del Portillo P, Robledo J, Zambrano MM. Respiratory tract clinical sample selection for microbiota analysis in patients with pulmonary tuberculosis. Microbiome. 2014 Aug 25;2:29. https://doi.org/10.1186/2049-2618-2-29.
  8. Fredre GD da, Filho FA, Ribeiro TS, Santos EO, Andrioli JL, Silva FL (2021). Conidiobolomycosis in Ovine in Southeast Bahia - Brazil. Acta Scientiae Veterinariae, 49(March):4–9. https://doi.org/10.22456/1679-9216.106793
  9. Fredriks D, Grissett-Hardwick G, Baumgartner W (2023). Nasal trichosporonosis in 2 mixed-breed ewes. Journal of Veterinary Diagnostic Investigation, 35(5):559–562. https://doi.org/10.1177/10406387231185568
  10. Helke KL, Meyerholz DK, Beck AP, Burrough ER, Derscheid RJ, Lohr C, McInnes EF, Scudamore CL, Brayton CF (2021). Research Relevant Background Lesions and Conditions: Ferrets, Dogs, Swine, Sheep, and Goats. ILAR Journal, 62(1–2):133–168. https://doi.org/10.1093/ilar/ilab005
  11. Turkar S, Devi S (2024). Diseases of respiratory system of goats. Trends in Clinical Diseases, Production and Management of Goats, 283–297.https://doi.org/10.1016/B978-0-443-23697-6.00020-2
  12. Clercx C (2022). Respiratory disorders. In Clinical Medicine of the Dog and Cat (pp. 188–219). CRC Press. https://doi.org/10.1201/b19610
  13. Ishak SR, Abd El Sayed STK, Wahba NS (2020). Prevalence of common sensitizing aeroallergens in Egyptian asthmatic patients. World Allergy Organization Journal, 13(4):100115. https://doi.org/10.1016/j.waojou.2020.100115
  14. Ugochukwu ICI, Aneke CI, Sani NA, Omeke JN, Anyanwu MU, Odigie AE, Onoja RI, Ocheja OB, Ugochukwu MO, Luca I, Makanju OA (2022). Important Mycoses of Wildlife: Emphasis on Etiology, Epidemiology, Diagnosis, and Pathology—A Review: PART 1. Animals, 12(15):1–19. https://doi.org/10.3390/ani12151874
  15. Guo W, Wang W, Bi S, Long R, Ullah F, Shafiq M, Zhou M, Zhang Y (2020). Characterization of anaerobic rumen fungal community composition in yak, Tibetan sheep and small tail han sheep grazing on the Qinghai-Tibetan Plateau. Animals, 10(1):144. https://doi.org/10.3390/ani10010144
  16. Wanjala G, Astuti PK, Bagi Z, Kichamu N, Strausz P, Kusza S (2023). A review on the potential effects of environmental and economic factors on sheep genetic diversity: Consequences of climate change. Saudi Journal of Biological Sciences, 30(1):103505. https://doi.org/10.1016/j.sjbs.2022.103505
  17. Vangrinsven E, Fastrès A, Taminiau B, Billen F, Daube G, Clercx C (2023). Assessment of the nasal microbiota in dogs with fungal rhinitis before and after cure and in dogs with chronic idiopathic rhinitis. BMC Microbiology, 23(1):1–16. https://doi.org/10.1186/s12866-023-02828-7
  18. Lahouar A, Marin S, Crespo-Sempere A, Saïd S, Sanchis V (2016). Efectos de la temperatura, la actividad de agua y el tiempo de incubación en el crecimiento fúngico y la producción de aflatoxina B1 por aislados toxicogénicos de Aspergillus flavus en sorgo. Revista Argentina de Microbiología, 48(1):78–85. http://dx.doi.org/10.1016/j.ram.2015.10.001
  19. Heaton SM, Weintrob AC, Downing K, Keenan B, Aggarwal D, Shaikh F, Tribble DR, Wells J, Group IDCRPTIDOS (2016). Histopathological techniques for the diagnosis of combat-related invasive fungal wound infections. BMC Clinical Pathology, 16:1–9. https://doi.org/10.1186/s12907-016-0033-9
  20. Seyedmousavi S, Bosco SMG de, De Hoog S, Ebel F, Elad D, Gomes RR, Jacobsen ID, Jensen HE, Martel A, Mignon B (2018). Fungal infections in animals: a patchwork of different situations. Medical Mycology, 56(suppl_1):S165–S187. https://doi.org/10.1093/mmy/myy028
  21. Kidd S, Halliday C, Ellis D (2016). Descriptions of medical fungi. CABI. https://doi.org/10.1079/9781800622340.0000
  22. Stecher G, Tamura K, Kumar S (2020). Molecular evolutionary genetics analysis (MEGA) for macOS. Molecular Biology and Evolution, 37(4):1237–1239. https://doi.org/10.1093/molbev/msz312
  23. Steyl RL (2017). Mycotic rhinitis in a Mutton Merino ewe. Journal of the South African Veterinary Association, 88(1):6–8. https://doi.org/10.4102/jsava.v88i0.1429
  24. Macias-Valle L, Finkelstein-Kulka A, Manji J, Okpaleke C, Al-Salihi S, Javer AR. Evaluation of sheep sinonasal endoscopic anatomy as a model for rhinologic research. World J Otorhinolaryngol Head Neck Surg. 2018 May 25;4(4):268-272. https://doi.org/10.1016/j.wjorl.2018.05.002.
  25. Enache I, Ioniță E, Mitroi M, Anghelina F, Mogoantă C, Ciolofan S, Căpitănescu A, Stepan A, Simionescu C (2020). Histopathological features of chronic rhinosinusitis with nasal allergic polyps. Current Health Sciences Journal, 46(1):66. https://doi.org/10.1177/1040638720958338
  26. Latgé J-P (1999). Aspergillus fumigatus and aspergillosis. Clinical Microbiology Reviews, 12(2):310–350. https://doi.org/10.1128/cmr.12.2.310
  27. Ribes JA, Vanover-Sams CL, Baker DJ (2000). Zygomycetes in human disease. Clinical Microbiology Reviews, 13(2):236–301. https://doi.org/10.1128/cmr.13.2.236
  28. White TJ, Bruns T, Lee S, Taylor J (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide to Methods and Applications, 315-22.https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  29. Richardson M, Rautemaa-Richardson R (2019). Exposure to Aspergillus in home and healthcare facilities' water environments: Focus on biofilms. Microorganisms, 7(1):7.https://doi.org/10.3390/microorganisms7010007

 

 

Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 24, Issue 1
January 2025
Pages 55-62
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