In sheep farming, one of the most crucial economic characteristics is increasing the number of livestock wondering for female newborns. This study aimed to investigate the middle-aged Iraqi sheep breed and determine its correlation with litter size by employing three distinct synchronization methods on the reproductive capabilities of Iraqi ewes grown in mid-southern Iraq. In a randomized block experimental design, eighty local ewes, aged three to four years old, were divided into four groups for the purpose of estrus synchronization: group 1 (n = 20 control animals), group 2 (n = 20 Medroxy Progesterone Acetate (MAP) sponges only), group 3 (n = 20 MAP sponges and GnRH), and group 4 (n = 20 MAP sponge and equine chorionic gonadotropin (eCG)). First, an ANOVA was applied to the data on estrus response, insemination, pregnancy, and prolificacy, followed by a chi square test. At (p≤0.05), the results were deemed significant. Over 80% of the ewes tested were response positively for estrus according to the group 2, which showed an intriguing success estrus rate. More than 75% of the ewes examined in Group 3 procedure demonstrated estrus. When the procedures were compared, group 3 outcomes for positive estrus, pregnancy rates, and litter size were superior. Therefore, it can be said that all tested protocols yielded outcomes that were good in terms of prolificacy, or the number of lambs produced per ewe, and estrus manifestation in compared to control group. While other treatments with the presence of the ram effect may improve the lambing rate and raise litter size, the MAP only protocol demonstrated higher findings for positive indicator of female pregnancy 66.6%.
Pedrosa VB, Santana Junior ML, Oliveira PS, Eler JP, Ferraz JBS. Population structure and inbreeding effects on growth traits of Santa Inês sheep in Brazil. Small Rumin Res. 2010;93:135-139. https://doi.org/10.1016/j.smallrumres.2010.05.012
Karam KM. A study on plasma estradiol and progesterone profile at days 11, 12 and 13 post ovulation undergone embryo transfer at day 7-post ovulation in local Egyptian mare. Al-Qadisiyah J Vet Med Sci. 2017;16(1).https://doi.org/10.29079/vol16iss1art47
Cavalcanti AS, Brandão FZ, Nogueira LAG, Fonseca JF. Effects of GnRH administration on ovulation and fertility in ewes subjected to estrous synchronization. Rev Bras Zootec. 2012;41(6):1412-1418.https://doi.org/10.1590/S1516-35982012000600014
Ezzat AA, Ahmed MN, Elabdeen MAEZ, Sabry AM. Estrus synchronization in Ossimi sheep by progestins. Alexandria J Vet Sci. 2016;51(1):207-214.
Zonturlu AK, Özyurtlu N, Kaçar C. Effect of different doses PMSG on estrus synchronization and fertility in Awassi ewes synchronized with progesterone during the transition period. Kafkas Univ Vet Fak Derg. 2011;17(1):125-129.
Abdalla EB, Farrag B, Hashem ALS, Khalil FA, Abdel-Fattah MS. Effect of progestagen, PGF2α, PMSG and GnRH on estrus synchronization and some reproductive and productive traits in Barki ewes. J Agroaliment Process Technol. 2014;20(1):93-101.
Najafi G, Cedden F, Maleki SA. The determination of plasma progesterone, estradiol-17β hormone levels in Ghezel sheep treated with CIDR and various doses of PMSG during the breeding season. Bull Environ Pharmacol Life Sci. 2014;3:118-122.
Martemucci G, D'Alessandro AG. Estrous and fertility responses of dairy ewes synchronized with combined short-term GnRH, PGF2α and estradiol benzoate treatments. Small Rumin Res. 2010;93:41-47.https://doi.org/10.1016/j.smallrumres.2010.05.001
Wildeus S. Current concepts in synchronization of estrus: sheep and goats. J Anim Sci. 2000;77(1):1-14.https://doi.org/10.2527/jas2000.00218812007700ES0040x
Metodiev N. Estrus synchronization of ewes by using "ram effect" and single treatment with synthetic analogue of PGF2α. Bulg J Agric Sci. 2015;21:889-892.
Abecia JA, Forcada F, Gonzáles-Bulnes A. Pharmaceutical control of reproduction in sheep and goats. Vet Clin North Am Food Anim Pract. 2011;27(1):67-79.https://doi.org/10.1016/j.cvfa.2010.10.001
Beilby KH, Grupen CG, Thomson PC, Maxwell WM, Evans G. The effect of insemination time and sperm dose on pregnancy rate using sex-sorted ram sperm. Theriogenology. 2009;71(5):829-835.https://doi.org/10.1016/j.theriogenology.2008.10.005
Abecia JA, Forcada F, Gonzalez-Bulnes A. Hormonal control of reproduction in small ruminants. Anim Reprod Sci. 2012;130:173-179.https://doi.org/10.1016/j.anireprosci.2012.01.011
Kuru M, Öğün M, Kulaksiz R, Kükürt A, Oral H. Comparison of oxidative/nitrosative stress, leptin and progesterone concentrations in pregnant and non-pregnant Abaza goats synchronized with controlled internal drug release application. Kafkas Univ Vet Fak Derg. 2018;24:887-892.
Kuru M, Boga Kuru B, Sogukpinar O, Cebi Sen C, Oral H, Kirmizibayrak T. Oestrus synchronization with progesterone-containing sponge and equine chorionic gonadotropin in Pirlak ewes during the non-breeding season: Can Toryum improve fertility parameters? J Vet Res. 2020;64:573-579.https://doi.org/10.2478/jvetres-2020-0074
Kuru M, Merhan O, Kaya S, Oral H, Kukurt A. The effect of short-term progesterone-releasing intravaginal device treatment on acute inflammation markers for Holstein heifers. Rev Med Vet. 2015;166:336-340.
Garoussi MT, Mavadati O, Bahonar M, Ragh MJ. The effect of medroxyprogesterone acetate with or without eCG on conception rate of fat-tail ewes in out of breeding season. Trop Anim Health Prod. 2020;52:1617-1622.https://doi.org/10.1007/s11250-019-02159-8
Powell MR, Kaps M, Lamberson WR, Keisler DH. Use of melengestrol acetate-based treatments to induce and synchronize estrus in seasonally anestrous ewes. J Anim Sci. 1996;74:2292-2302.https://doi.org/10.2527/1996.74102292x
Arya D, Goswami R, Sharma M. Estrous synchronization in cattle, sheep and goat. Multidiscip Rev. 2023;6(1):2023001.https://doi.org/10.31893/multirev.2023001
Pellicer-Rubio MT, Laignel G, Thomas Y, Prache S, Benoit M, Tournadre H. Reproductive performance in two organic sheep farming systems differing by the number of mating sessions in and out of the breeding season. Theriogenology. 2023;195:238-248.https://doi.org/10.1016/j.theriogenology.2022.10.035
Dzomba EF, Chimonyo M, Snyman MA, Muchadeyi FC. The genomic architecture of South African mutton, pelt, dual-purpose and nondescript sheep breeds relative to global sheep populations. Anim Genet. 2020;51:910-923.https://doi.org/10.1111/age.12991
Wanjala G, Kusuma Astuti P, Bagi Z, Kichamu N, Strausz P, Kusza S. A review on the potential effects of environmental and economic factors on sheep genetic diversity: Consequences of climate change. Saudi J Biol Sci. 2023;30:103505.https://doi.org/10.1016/j.sjbs.2022.103505
Janssens S, Vandepitte W, Bodin L. Genetic parameters for litter size in sheep: natural versus hormone-induced oestrus. Genet Sel Evol. 2004;36:543-562.https://doi.org/10.1186/1297-9686-36-5-543
Kuru M, Sogukpinar O, Makav M, Cetin N. Effect of barium selenate injections on fertility of Pirlak ewes subjected to estrus synchronization during non-breeding season. Med Weter. 2017;73:479-482.https://doi.org/10.21521/mw.5758
Kuru M, Oral H, Kulaksiz R. Determination of gestational age by measuring defined embryonic and foetal indices with ultrasonography in Abaza and Gurcu goats. Acta Vet Brno. 2018;87:357-362.https://doi.org/10.2754/avb201887040357
Martínez-Ros P, González-Bulnes A. Efficiency of CIDR-based protocols including GnRH instead of eCG for estrus synchronization in sheep. Animals. 2019;9(4):146.https://doi.org/10.3390/ani9040146
Santos-Jimenez Z, et al. Comparative efficiency of oestrus synchronization in sheep with progesterone/eCG and progesterone/GnRH during breeding and non-breeding season. Reprod Domest Anim. 2020;55(7):882-884.https://doi.org/10.1111/rda.13698
Dursun Ş. Gonadotrophin stimulation of ewes that are not pregnant following multiple matings during the season. Turk J Vet Anim Sci. 2019;43(1):5.https://doi.org/10.3906/vet-1806-22
Mottershead D, et al. Cumulin, an oocyte-secreted heterodimer of the transforming growth factor-β family, is a potent activator of granulosa cells and improves oocyte quality. J Biol Chem. 2015;290(39):24007-24020.https://doi.org/10.1074/jbc.M115.671487
Bodin L, et al. A novel mutation in the bone morphogenetic protein 15 gene causing defective protein secretion is associated with both increased ovulation rate and sterility in Lacaune sheep. Endocrinology. 2007;148(1):393-400.https://doi.org/10.1210/en.2006-0764
Monteagudo L, et al. A 17bp deletion in the Bone Morphogenetic Protein 15 (BMP15) gene is associated with increased prolificacy in the Rasa Aragonesa sheep breed. Anim Reprod Sci. 2009;110(1-2):139-146.https://doi.org/10.1016/j.anireprosci.2008.01.005
Ayoub MA, Tharwat RAM, Yaseen MA, Soliman ES. Using progesterone and prostaglandin F2α for ewes' estrus synchronization during summer season in Egypt. J Anim, Poultry Fish Prod. 2020;9(1):1-8.https://doi.org/10.21608/japfp.2020.119466
Gardón JC, Escribano B, Astiz S, Ruiz S. Synchronization protocols in Spanish Merino sheep: Reduction in time to estrus by the addition of eCG to a progesterone-based estrus synchronization protocol. Anim Sci. 2015;15(2):409-418.https://doi.org/10.1515/aoas-2015-0003
Abu EL-Ella AA, Teleb DF, Abdel-Hafez MAM, Deghedy AM. Appraisal of different protocols for estrus synchronization in local Rahmani sheep. Egypt J Sheep Goat Sci. 2016;11(3):116-131.https://doi.org/10.12816/0035352
Azawi OI, Al-Mola MKM. Laparoscopic intrauterine insemination of Awassi ewes superovulated with equine chorionic gonadotropin. Al-Qadisiyah J Vet Med Sci. 2010;9(2):1-5.https://doi.org/10.29079/vol9iss2art97
Omar AA. Progesterone and estrogen levels in Awassi ewes treated with vaginal sponges. J Vet Med Sci. 2013;12(1):183-186.https://doi.org/10.29079/vol12iss1art247
Hameed,A and Karam,K Mohammed. (2025). Effect of utilizing different synchronizing protocols on reproductive efficiency of local Iraqi sheep. Al-Qadisiyah Journal of Veterinary Medicine Sciences, 24(1), 1-6. doi: 10.29079/qjvms.2024.149988.1017
MLA
Hameed,A , and Karam,K Mohammed. "Effect of utilizing different synchronizing protocols on reproductive efficiency of local Iraqi sheep", Al-Qadisiyah Journal of Veterinary Medicine Sciences, 24, 1, 2025, 1-6. doi: 10.29079/qjvms.2024.149988.1017
HARVARD
Hameed A, Karam K Mohammed. (2025). 'Effect of utilizing different synchronizing protocols on reproductive efficiency of local Iraqi sheep', Al-Qadisiyah Journal of Veterinary Medicine Sciences, 24(1), pp. 1-6. doi: 10.29079/qjvms.2024.149988.1017
CHICAGO
A Hameed and K Mohammed Karam, "Effect of utilizing different synchronizing protocols on reproductive efficiency of local Iraqi sheep," Al-Qadisiyah Journal of Veterinary Medicine Sciences, 24 1 (2025): 1-6, doi: 10.29079/qjvms.2024.149988.1017
VANCOUVER
Hameed A, Karam K Mohammed. Effect of utilizing different synchronizing protocols on reproductive efficiency of local Iraqi sheep. QJVMS. 2025;24(1):1-6. doi: 10.29079/qjvms.2024.149988.1017