A total of 60 one-day-old Ross broiler chicks were used in this investigation. The birds were categorized into four groups, with each group including 15 birds. Group A was administered a 0.5 ml dosage of the LaSota live vaccination against Newcastle disease using the oral approach on the first day of their lives, using a 1 ml syringe. On the other hand, Group B got the dead vaccine by a subcutaneous injection. Group C was administered two distinct forms of vaccine: live and dead, whilst Group D was designated as the control group. On the 35th day, the birds were euthanized in order to obtain blood samples for the purpose of studying the impact of various vaccination types on the immunological response to the ND vaccine. The response was evaluated using an ELISA test. The findings demonstrated statistically significant differences (P≤0.05) in antibody titers between group B and the control group. Additionally, groups C and A exhibited substantial increases (p<0.05) in antibody titers compared to the control group, which experienced a significant drop (P≤0.05) in antibody titers. Overall, the results indicated that the inactivated vaccine produced a higher level of antibodies. However, further evidence demonstrated that administering a live virus vaccination on the first day after birth resulted in the suppression of the mother's immune response by the vaccine virus. Hence, it is crucial to ascertain the antibody titer prior to determining the vaccine's course.