Al-Qadisiyah Journal of Veterinary Medicine Sciences

The activity of three different propolis extracts and their antioxidant activity induced by CCL4 in adult rats

Document Type : Research Paper

Authors

1 Unit of Zoonotic Diseases, College of Veterinary medicine, University of Al-Qadisiyah, Al-Diwaniyah City, Iraq.

2 Unit of zoonotic disease / college of veterinary medicine/ University of Al-Qadisiyah

Abstract

The goal of the current study was to examine the impact of extracting propolis in various solvents in light of the antioxidant activity induced by CCL4. The biological study was conducted using 25 adult Wistar male rats. Five rats served as normal control group which fed on basal diet with drinking tap water. While, other 20 rats received intra-peritoneal single dose of 0.5 ml/kg CCl4 to induce renal and hepatic oxidative stress. Then, rats reclassified into 4 groups (n=5), positive control and the other three groups were treated by watery, ethanolic and ethyl acetate extracts of propolis instead of water till the end of experimental period (9 days). In vivo extracts evaluation includes serum Catalase, SOD, GSH, TAOC, and MDA were measured at the end of the experiment. The In vitro results showed all extracts exhibited increasing radical scavenging activities with increased concentrations of extract with variation in antioxidant activity according to the type of extract. The CCL4 group rats showed significant (P<0.05) decline in the catalase, SOD, GSH and TAOC and significant (P<0.05) evaluation in the MDA compared with control. All prepared extracts corrected the deviation occurring in the parameters as a result of administering the CCL4, with variation depending on the type of extract. Watery extract showed best results in enhance catalase level, while ethanolic extract showed best results in enhance GSH activity, no difference in the SOD, T-AOC and MDA between watery and ethanolic extracts, in the same time the ethyl acetate extract was less effective. The In vitro and In vivo antioxidant activity was varied according to the type of extract; ethanol gave best activity.

Keywords

References
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Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 23, Issue 1
May 2024
Pages 46-56
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