Al-Qadisiyah Journal of Veterinary Medicine Sciences
Official Journal of the College of Veterinary Medicine, University of Al-Qadisiyah

Molecular Detection of Staphylococcus spp in Ovine Actinomycosis

Document Type : Research Paper

Authors

Raeda Nema Khadim 1
Saad Hashim Al-Husseiny 2

1 Internal and preventive medicine Department, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq

2 dept. of internal and preventive medicine, college of veterinary medicine, university of Al-qadisiyah, Iraq

https://doi.org/10.29079/qjvms.2024.185131
Abstract
Ovine actinomycosis, also referred to as lumpy jaw, is a multifactorial illness that is characterized by a number of host-pathogen-environment interactions in which host immune and physiological processes (host) interact with a variety of causative agents, such as staphylococci spp. Oral infections are common in sheep. Periodontal disease, one of the most common diseases, can start early with periodontitis and progress to become a chronic condition that causes food debris to build up around the tooth below the level of the gums. Staphylococcus spp. are widely dispersed in the environment and is primarily discovered as a commensally micro-flora on the skin of animals and birds as well as the mucous membranes, particularly the nasal cavity. The PCR assay was applied for the detection of Staphylococcus aureus and Staphylococcus haemolyticus from three ovine actinomycosis cases based on the amplification of the 16S rRNA gene. In addition, the diagnosis depended on sequencing of the PCR products, and gene analysis was done to match the global isolates. The current study found that Staphylococcus aureus was isolated from all samples used 86 samples and that gene sequencing of this strain confirmed 99.88% global compatibility, whereas Staphylococcus haemolyticus revealed 100% global compatibility. Therefore, the aims of the study were to pinpoint Staphylococcus spp. as the secondary causative agent of lumpy jaw disease in Awasii sheep of Iraq.

Keywords

Lumpy jaw
ovine
staphylococcus sp
Crossmark
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Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 23, Issue 1
May 2024
Pages 41-45

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