Document Type : Research Paper

Authors

1 Internal and preventive medicine Department, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq

2 dept. of internal and preventive medicine, college of veterinary medicine, university of Al-qadisiyah, Iraq

Abstract

Ovine actinomycosis, also referred to as lumpy jaw, is a multifactorial illness that is characterized by a number of host-pathogen-environment interactions in which host immune and physiological processes (host) interact with a variety of causative agents, such as staphylococci spp. Oral infections are common in sheep. Periodontal disease, one of the most common diseases, can start early with periodontitis and progress to become a chronic condition that causes food debris to build up around the tooth below the level of the gums. Staphylococcus spp. are widely dispersed in the environment and is primarily discovered as a commensally micro-flora on the skin of animals and birds as well as the mucous membranes, particularly the nasal cavity. The PCR assay was applied for the detection of Staphylococcus aureus and Staphylococcus haemolyticus from three ovine actinomycosis cases based on the amplification of the 16S rRNA gene. In addition, the diagnosis depended on sequencing of the PCR products, and gene analysis was done to match the global isolates. The current study found that Staphylococcus aureus was isolated from all samples used 86 samples and that gene sequencing of this strain confirmed 99.88% global compatibility, whereas Staphylococcus haemolyticus revealed 100% global compatibility. Therefore, the aims of the study were to pinpoint Staphylococcus spp. as the secondary causative agent of lumpy jaw disease in Awasii sheep of Iraq.

Keywords

  1. Casey A, Lambert PA, Elliott T. Staphylococci. Int J Antimicrob Agents. 2007;29:S23-32. https://doi.org/10.1016/S0924-8579(07)72175-1
  2. Kérouanton A, Hennekinne J, Letertre C, Petit L, Chesneau O, Brisabois A, et al. Characterization of Staphylococcus aureus strains associated with food poisoning outbreaks in France. Int J Food Microbiol. 2007;115:369-75. https://doi.org/10.1016/j.ijfoodmicro.2006.10.050
  3. Pereira V, Lopes C, Castro A, Silva J, Gibbs P, Teixeira P. Characterization for enterotoxin production, virulence factors, and antibiotic susceptibility of Staphylococcus aureus isolates from various foods in Portugal. Food Microbiol. 2009;26:278-82. https://doi.org/10.1016/j.fm.2008.12.008
  4. Abulreesh HH. Multidrug-resistant staphylococci in the environment. In: Proceedings of the International Conference on Biotechnology and Environment Management; 2011. Vol. 18, pp. 1-6. https://doi.org/10.3923/jm.2011.510.523
  5. Suleiman A, Zaria L, Grema H, Ahmadu P. Antimicrobial resistant coagulase positive Staphylococcus aureus from chickens in Maiduguri, Nigeria. Sokoto J Vet Sci. 2013;11:51-5. https://doi.org/10.4314/sokjvs.v11i1.8
  6. Cremonesi P, Perez G, Pisoni G, Moroni P, Morandi S, Luzzana M, et al. Detection of enterotoxigenic Staphylococcus aureus isolates in raw milk cheese. Lett Appl Microbiol. 2007;45:586-91. https://doi.org/10.1111/j.1472-765X.2007.02231.x
  7. Ippolito G, Leone S, Lauria FN, Nicastri E, Wenzel RP. Methicillin-resistant Staphylococcus aureus: the superbug. Int J Infect Dis. 2010;14:S7-11. https://doi.org/10.1016/j.ijid.2010.05.003
  8. McGavin MD, Carlton WW, Zachary JF. Thomson's special veterinary pathology. 3rd ed. St Louis: Mosby; 2001. p. 325-45.
  9. Ruiz de Arcaute M, Ferrer LM, Lacasta D, González JM, De las Heras M, Borobia M, et al. Prevalence of dental and mandibular disorders in culled sheep in Spain. Aust Vet J. 2020;98:438-41. https://doi.org/10.1111/avj.12990
  10. Ruiz de Arcaute M, Ferrer LM, Lacasta D, Gonzalez JM, De Las Heras M, Borobia M, et al. Prevalence of dental and mandibular disorders in culled sheep in Spain. Aust Vet J. 2020;98:438-41. https://doi.org/10.1111/avj.12990
  11. Ramesh V, Ganti SR, Gattu S, Sharma R. Rare and unexpected cause for retropharyngeal abscess in an immunocompetent man: metastatic community-acquired methicillin-resistant Staphylococcus aureus infection. BMJ Case Rep CP. 2023;16:e254785. https://doi.org/10.1136/bcr-2023-254785
  12. Pal M, Shuramo MY, Tewari A, Srivastava JP, HD C. Staphylococcus aureus from a Commensal to Zoonotic Pathogen: A Critical Appraisal.
  13. Pal M, Kerorsa GB, Marami LM, Kandi V. Epidemiology, pathogenicity, animal infections, antibiotic resistance, public health significance, and economic impact of staphylococcus aureus: a comprehensive review. Am J Public Health Res. 2020;8:14-21.
  14. Pal M, Gutama KP, Koliopoulos T. Staphylococcus aureus, an important pathogen of public health and economic importance: A comprehensive review. J Emerg Environ Technol Health Prot. 2021;4:17-32.