Authors

• Wisam Hussein Selman ¹
• Farid Jamil Al-Tahan ²

¹ College of Veterinary Medicine, University of Al-Qadisiyah

² College of Veterinary Medicine, University of Baghdad

Abstract

The present study was designed to determine the effect of the aqueous extract of powdered Nigella sativa seeds on pre-contracted smooth muscle isolated from the trachea in sheep.Parts of the trachea were taken from freshly slaughtered sheep and were kept viable in Krebs–Henseleit solution at (37 C),then the smooth muscles were isolated from the trachea and proceeded for studying its contraction in physiograph. Aqueous extract of Nigella sativa seeds was prepared and six concentrations of the aqueous extract were used (50, 100, 150, 200, 250, and 300 mg / 10 ml). The smooth muscle was stimulated for contraction with different drugs (acetylcholine or histamine). Next, one milliliter of the prepared six serially increasing concentrations of the Nigella sativa aqueous extract was added separately to pre-contracted isolated tracheal smooth muscle to determine any probable inhibitory effect of the seeds extract. Aqueous extract of Nigella sativa seeds caused relaxation of tracheal smooth muscle pre-contracted with acetylcholine or histamine which may have taken place through stimulation of the non-adrenergic – non-cholinergic system.

 

Keywords

• Nigella sativa
• isolated trachea
• sheep
• smooth muscle