Authors
Department of Physiology and Pharmacology, Veterinary Medicine Collage, Al-Qadisiyah University, Iraq
Abstract
Objective :The current study aimed to investigate the possibility of using nano-piperine to reduce the toxic effects of sodium benzoate on the female reproductive system of female rats . Methods: fourty female Wister rats with an average weight of 150 ± 10 g were used, dosed for 30 consecutive days they divided randomly into four equal groups (10 per group). The animals were allowed to acclimatize for 14 days before the experiment . The first group (C) was given 1 ml of distilled water orally as a control group. The second group (T1) was given sodium benzoate orally in a dose of 100 mgkg B.W. The third group (T2) was given Nano-piperine orally in a dose of 25 mg /kg /B.W. The fourth group (T3) was given both sodium benzoate and Nano-piperine at the same time . After the end of treatments , ovary and uterus samples were removed from all animals and reserved them in formalin 10% for histological examination Results: The result of our study showed follicular inhibition and congestion of ovarian stroma in T1 group when compared with the control group . The structure of ovary in T2 group is similar to that of the control group . In T3 group the structure of the ovary looked almost normal , and increase in follicular growth wave when compared with the T1 group . The result of the uterine sections indicated degeneration and vacuolation of epithelial cells and complete absence of uterine gland ,. and infiltration of inflammatory cells in the endometrium . On the other hand , uterus section in T2 group revealed normal uterus cells without any significant lesion . while the uterine tissue in animals of T3 group showed a hyperplasia in epithelial cells , and proliferation of uterine glands and normal columnar epithelium of uterus .Conclusion :Nano-piperine at dose of 25 mg/kg has the ability to reduce histological changes on the female reproductive system resulting from treatment with sodium benzoate .
Keywords
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