Al-Qadisiyah Journal of Veterinary Medicine Sciences

Histopathological study of the effect of treatment with Nigella sativa against Iron stress in liver

Document Type : Research Paper

Authors

Department of physiology College of Veterinary Medicine University of AI-Qadisiyah, Iraq

10.29079/qjvms.2022.179329

Abstract

This experiment was conducted for the purpose of knowing the impact of antioxidant nanoparticles of Nigella sativa agonist oxidative stress of the Nanoparticles the iron in rats .the experiment was done in veterinary medicine Al-Qadisiyah university at the animal house .This study was carried out during a period from 20.11.2020 to 20.04.2021 (64) sixty four mature rat 90 days age , average body weight (100-120) gram were used in the current study the rat were kept under controlled hygienic condition with free access to food and water for two week before starting the experiment. The animal were randomly assigned into 4equal groups 16 each and treated as follow. Experimental design :-first group(control) :will be kept without treatment as negative control .Second group (IONP) :Will be orally administered with IONPs (10 mgkgday) for 28 days .Third group (NSMNP) : Will be orally administered with NSMNPSs(25 mg Kg ) for 28 day .Forth group (IO+NSMNP) mix : will be orally administered with combination of IONPs(10mgkgday) and NSMNPs (25mg kg day)for 28day.Each group will be allocated to two subgroup as follow: Subgroup 1:8 male rats will be sacrificed after 14 days of treatment .Subgroup 2:8 male rats will be sacrificed after 28 days of treatment. The following objective will be studied ,and the most important changes observed Histological examination when administration after 14 and 28 days administration Nanoparticles showed on liver slid Iron loss hepatic cords necrosis hepatocytes and around central vein, congestion blood vessel inflammatory cells macrophages ,hyperplasia in bile duct ,congestion bile duct ,marked of dilation of sinusoids, hemorrhage within the hepatic tissue ,proliferation of kupffer cells ,binucleated hepatocytes compared with control. mix groups(IO+NSMNP) showed marked mitotic division of hepatocytes which showed as binucleated ,Congestion and dilation of sinusoids with proliferation of Kupffer cells ,showed of radially arrangement of hepatocytes around of central vein, appearance (hexagonal shape with prominent &central nuclei) and few showed as binuleated hepatocytes .Dilation of sinusoids and present of Kupffer cells . with scattered and mild infiltration of macrophages and few congestion in the blood vessels .The hepatocytes showed with normal shape, compared with control. When administration Nanoparticles Nigella sativa normal Gross section obtained from liver tissue normal radially arrangement of hepatic cords around normal central vein. Binuleated hepatic cords around normal central vein. Binuleated hepatocytes, compared with control.

Keywords

References
1. Han X, Li S, Peng Z, Al-yuobi ASO, El-Shahawi MS, Leblanc RM. Interactions between carbon nanomaterials and biomolecules. J Oleo Sci. 2016;65:1-7.https://doi.org/10.5650/jos.ess15248
2. Raza MA, Rauf Z, Sabi AN, Rias S, Naseem. Size and shape dependent antibacterial of silver nano. 2016;4:74.https://doi.org/10.3390/nano6040074
3. Cacciotti I, Chronopoulou L, Palocci C, Amalfitano A, Cantiani Cordaro M, Lajolo C, et al. Controlled release of 18-β glycyrrhetic acid by nano delivery systems increases cytotoxicity on oral carcinoma cell line. Nanotechnology. 2018;29(28):285101.https://doi.org/10.1088/1361-6528/aabecc
4. Dwivedi SN. Herbal remedies among tribes of Siddhi district of Madhya Pradesh. J Econ Tax. 2004;28(3):675-686.
5. Nickavar B, Mojab F, Javidnia K, Amoli MA. Chemical composition of the fixed and volatile oils of Nigella sativa L. from Iran. Z Naturforsch C. 2003;58(9-10):629-631.https://doi.org/10.1515/znc-2003-9-1004
6. Ali BH, Blunden G. Pharmacological and toxicological properties of Nigella sativa. Phytother Res. 2003;17:299-305.https://doi.org/10.1002/ptr.1309
7. Al-Ghandi MS. Protective effect of Nigella sativa seeds against carbon tetrachloride-induced liver damage. Am J Chin Med. 2003;31(5):721-728.https://doi.org/10.1142/S0192415X03001399
8. Kalus U, Pruss A, Bystron J, Jureck M, Smekalova A, Lichius J, et al. Effect of Nigella sativa (black seed) on subjective feeling in patients with allergic disease. J Phytother Res. 2003;17(10):1209-1214.https://doi.org/10.1002/ptr.1356
9. Salem ML. Immunomodulatory and therapeutic properties of the Nigella sativa L. seed. Int Immunopharmacol. 2005;5:1749-1770.https://doi.org/10.1016/j.intimp.2005.06.008
10. Morsi N. Antimicrobial effect of crude extracts of Nigella sativa on multiple antibiotics-resistant bacteria. J Acta Microbiol Colonic. 2000;49(1):63-74.
11. Riaz M, Syed M, Chaudhary FM. Chemistry of medicinal plants of the genus Nigella (Family Ranunculaceae). J Hand Medicus. 1996;39(2):40-45.
12. Musa D, Duisiz N, Gumushan H, Ulakoglu G, Muharrem. Antitumor activity of ethanol extract of Nigella sativa seed. J Biol Bratislava. 2004;59:635-670.
13. Al-Rowais NA. Herbal medicine in the treatment of diabetes mellitus. Saudi Med J. 2002.
14. Zaoui I, Cherrah Y, Mahassini N, Alaoui K, Amar Ouch H, Hassar M. Acute and chronic toxicity of Nigella sativa fixed oil. J Phytomedicine. 2002;9(1):3-6.https://doi.org/10.1078/0944-7113-00084
15. Szalay B, Tatrai E, Nyiro G, Vezer T, Dura G. Potential toxic effects of iron oxide nanoparticles in vivo and vitro experiment. J Appl Toxicol. 2017;32(6):446-453.https://doi.org/10.1002/jat.1779
16. Harboron HJ. Phytochemical methods: A guide to modern techniques of plant analysis. Chapman and Hall, London, UK; 1984. p. 1-34.
17. Awan MA, Akhter S, Husna AU, Ansari MS, Rakha BA, Azam MA, Qadeer S. Antioxidant activity of Nigella sativa seeds aqueous extract and its use for cryopreservation of buffalo spermatozoa. Andrologia. 2018;50(6):e13020-e13026.https://doi.org/10.1111/and.13020
18. Amooaghaie R, Saeri MR, Azizi M. Synthesis, characterization and biocompatibility of silver nanoparticles synthesized from Nigella sativa leaf extract in comparison with chemical silver nanoparticles. Ecotoxicol Environ Saf. 2015;120:400-408.https://doi.org/10.1016/j.ecoenv.2015.06.025
19. Luna LG. Manual of histological staining methods of the Armed Forces Institute of Pathology. 3rd edition. McGraw Hill; 1968.
20. Ahmed A. Activation of the immune system of Anopheles gambiae against malaria parasite: A comparison between bacterial injection and botanical extract. Int Conf Biol Sci. 2004;3(1):122-141.
21. Ramadan M, Morsel J. Analysis of glycolipids from black cumin (Nigella sativa L.), coriander (Coriandrum sativum L.), and niger (Guizotia abyssinica Cass.) oil seeds. Food Chem. 2003;80:197-204.https://doi.org/10.1016/S0308-8146(02)00254-6
22. Swami SM, Tan BK. Cytotoxic and immunopotentiating effect of ethanolic extract of Nigella sativa L. seeds. J Ethnopharmacol. 2000;70:1-7.https://doi.org/10.1016/S0378-8741(98)00241-4
23. Salem M, Hossein M. Protective effect of black seed oil from Nigella sativa against murine cytomegalovirus infection. J Immunopharmacol. 2000;22(9):729-740.https://doi.org/10.1016/S0192-0561(00)00036-9
24. Andaloussi A, Elimadi A, Settaf A, Cherrah Y, Haddad P. The effect of Nigella sativa on body weight. J Ethnopharmacol. 2004;94:251-259.https://doi.org/10.1016/j.jep.2004.04.030
25. Kanter M, Coskun O, Gurel A. Effect of black cumin (Nigella sativa) on cadmium-induced oxidative stress in the blood of rats. J Biol Trace Elem Res. 2005;107(3):277-278.https://doi.org/10.1385/BTER:107:3:277
26. AItan M, Kanter M, Donmez S, Kartal M, Buyukbas S. Combination therapy of Nigella sativa and human parathyroid hormone on bone mass, biomechanical behavior and structure in streptozotocin-induced diabetic rats. Acta Histochem. 2007;4:304-314.https://doi.org/10.1016/j.acthis.2007.02.006
27. Latif M, Faheem M, Asmatullah, Hoseinifar SH, Doan HV. Animals. 2021;11:48.https://doi.org/10.3390/ani11010048
28. Ijaz H, Tulain UR, Qureshi J, Danish Z, Musayab S, Akhtar MF, Saleem A, Khan KK, Zaman M, Waheed I, et al. Nigella sativa (prophetic medicine): A review. Pak J Pharm Sci. 2017;30:229-234.
29. Cook JD. Adaptation in iron metabolism. Am J Clin Nutr. 1990;51:301-308.https://doi.org/10.1093/ajcn/51.2.301
30. Hussain M, Tunio AG, Akhtar L, Shaikh GS. Effects of Nigella sativa on various parameters in patients of non-alcoholic fatty liver disease. J Ayub Med Coll Abbottabad. 2017;29:403-407.
31. Shaik HS, Desai SD, Das KK, Haseena S. Hepatoprotective effect of Nigella sativa seed in streptozotocin-induced diabetic albino rats: histological observations. Int J Anat Res. 2016;4(2):2459-2463.https://doi.org/10.16965/ijar.2016.238
32. Devendna SK, Mond TS, Hemant KS, Ali A, Verma VK. In vivo hepatoprotective potential of Nigella sativa extract against rifampicin-induced sub-chronic hepatotoxicity and altered redox status. J Biol Act Prod Nat. 2012;2(3):167-177.https://doi.org/10.1080/22311866.2012.10719124
33. Salem ML. Immunomodulatory and therapeutic properties of the Nigella sativa L. seed. Int Immunopharmacol. 2005;5:1749-1770.https://doi.org/10.1016/j.intimp.2005.06.008
 
 
 
 
 
 
 
Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 21, Issue 1
February 2022
Pages 10-16
Files
Share
How to cite
Statistics