Authors
Department of physiology College of Veterinary Medicine University of AI-Qadisiyah, Iraq
Abstract
This experiment was conducted for the purpose of knowing the impact of antioxidant nanoparticles of Nigella sativa agonist oxidative stress of the Nanoparticles the iron in rats .the experiment was done in veterinary medicine Al-Qadisiyah university at the animal house .This study was carried out during a period from 20.11.2020 to 20.04.2021 (64) sixty four mature rat 90 days age , average body weight (100-120) gram were used in the current study the rat were kept under controlled hygienic condition with free access to food and water for two week before starting the experiment. The animal were randomly assigned into 4equal groups 16 each and treated as follow. Experimental design :-first group(control) :will be kept without treatment as negative control .Second group (IONP) :Will be orally administered with IONPs (10 mgkgday) for 28 days .Third group (NSMNP) : Will be orally administered with NSMNPSs(25 mg Kg ) for 28 day .Forth group (IO+NSMNP) mix : will be orally administered with combination of IONPs(10mgkgday) and NSMNPs (25mg kg day)for 28day.Each group will be allocated to two subgroup as follow: Subgroup 1:8 male rats will be sacrificed after 14 days of treatment .Subgroup 2:8 male rats will be sacrificed after 28 days of treatment. The following objective will be studied ,and the most important changes observed Histological examination when administration after 14 and 28 days administration Nanoparticles showed on liver slid Iron loss hepatic cords necrosis hepatocytes and around central vein, congestion blood vessel inflammatory cells macrophages ,hyperplasia in bile duct ,congestion bile duct ,marked of dilation of sinusoids, hemorrhage within the hepatic tissue ,proliferation of kupffer cells ,binucleated hepatocytes compared with control. mix groups(IO+NSMNP) showed marked mitotic division of hepatocytes which showed as binucleated ,Congestion and dilation of sinusoids with proliferation of Kupffer cells ,showed of radially arrangement of hepatocytes around of central vein, appearance (hexagonal shape with prominent ¢ral nuclei) and few showed as binuleated hepatocytes .Dilation of sinusoids and present of Kupffer cells . with scattered and mild infiltration of macrophages and few congestion in the blood vessels .The hepatocytes showed with normal shape, compared with control. When administration Nanoparticles Nigella sativa normal Gross section obtained from liver tissue normal radially arrangement of hepatic cords around normal central vein. Binuleated hepatic cords around normal central vein. Binuleated hepatocytes, compared with control.
Keywords
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