Al-Qadisiyah Journal of Veterinary Medicine Sciences

Turmeric extract ointment and diode laser for treatment of open wound

Document Type : Research Paper

Authors

Al-Qadisiyah Journal of Veterinary Medicine Sciences

10.29079/qjvms.2022.179326

Abstract

Objective: The study was aim to evaluate the effects of topical application of 20% turmeric extract ointment and 8J/cm2, 660nm λ diode laser therapy in treatment of open wounds in mice model. Methods: Thirty adult male mice, approximately weighing (50g), and aged (60-70 days) were utilized and randomly divided into two equal groups. Mice were anesthetized by IM injection mixture of Ketamine and Xylazine (80 mg/kg BW of Ketamine and 10 mg/kg BW of Xylazine). Animals were prepared for aseptic surgery, and then made two parallel vertical circular full thickness (0.5 cm) skin incisions on the back of each animal. G1 (control group) left without treatment. G2 (turmeric extract ointment + diode laser treatment group), in which the cranial wounds were treated with a topical application of 200 mg / cm² of 20% turmeric extract ointment in addition to 8J/cm2, 660nm λ diode laser treatment once daily for 7 successive days post wounding (PW), and the caudal wound was left without treatment as a control in the same animal. Wounds were evaluated both grossly and histopathological. For histopathological assessment, specimens were taken after 7, 14 and 21 days PW. Results: Grossly at 7, and 14 days, the size of the treated wounds in the treated group were significantly P < 0.05 smaller than that of the control group and control wounds of the same animal, whereas the readings converged at 21 days. Microscopically; G1 on day 7 exhibited thin early reepithelialization of epidermal layers, the dermis had developed new blood vessels, infiltration of inflammatory cells, and the dermo-epidermal interface had vacuolations. At 14 days, the epidermis developed stratum corneum hyperkeratosis, thin irregularly arranged re-epithelialized layers. Acantholysis, inflammatory cells, blood vessels, accumulation of fibroblasts and thick evenly collagen fibers in the dermis. G2 at 7 days PW displayed hyper cellular well-regenerated epithelial layers, and accumulation of collagen fibers, inflammatory cells, and fibroblasts in the dermis. At 14 days PW, thick well-differentiated epidermal layers resting on clear basement membrane were seen. In the dermis there were dense mature collagen fibers, numerous hair follicles and sebaceous glands. At 21 days PW, the regenerated epidermis was laying on the foundation membrane, giving it its typical look. The dermis layer also had a large number of hair follicles that are at various stages of development along with collagen fibers, and fibroblasts. Conclusions: Used of 20% turmeric extract ointment with diode laser had a synergistic effect on the healing of open skin wounds.

 

Keywords

References
  1. McGee HM, Schmidt BA, Booth CJ, Yancopoulos GD, Valenzuela DM, Murphy AJ, et al. IL-22 promotes fibroblast-mediated wound repair in the skin. J Investig Dermatol. 2012;463:1000-1038.
  2. Rodero MP, Khosrotehrani K. Skin wound healing modulation by macrophage. J Inter Clin Exp Patho. 2010;3:643-653.
  3. Swaim SF, Henderson JRRA. Small Animal Wound Management. 2nd ed. Williams and Wilkins; 1997. p. 27-29.https://doi.org/10.1111/j.1532-950X.1998.tb00113.x
  4. Li J, Chen J, Kirsner R. Pathophysiology of acute wound healing. J Clin Dermatol. 2007;25:9-18.https://doi.org/10.1016/j.clindermatol.2006.09.007
  5. Digelmann RF, Evans MC. An overview of acute fibrotic and delayed. J Front Bio Sci. 2004;9:283-289.https://doi.org/10.2741/1184
  6. Strodtbeck F. Physiology of wound healing. J Newborn Infant Nurs Rev. 2001;1:43-52.https://doi.org/10.1053/nbin.2001.23176
  7. Alam G, Singh MP, Singh A. Wound healing potential of some medicinal plants. J Inter Pharm Sci Rev Res. 2011;9:136-145.
  8. Stadelmann WK, Digenis A, Tobin G. Impediments to wound healing. J Am Surg. 1998;176:39-47.https://doi.org/10.1016/S0002-9610(98)00184-6
  9. Kumar V, Cortran R, Robbins S, editors. Basic pathology. 7th ed. Saunders; 2003. p. 873-891.
  10. Li B, Wang J. Fibroblast and myofibroblasts in wound healing: force generation and measurement. J Tissue Viability. 2011;20:108-120.https://doi.org/10.1016/j.jtv.2009.11.004
  11. Hawkins D, Abrahamse H. Effect of multiple exposure of low-level laser therapy on the cellular responses of wounded human skin fibroblasts. J Photomed Laser Surg. 2006;24:705-714.https://doi.org/10.1089/pho.2006.24.705
  12. Diwan MF, Abid TA. The effect of different doses of 660 nm laser therapy on open skin wound healing in Wistar rats. Kufa J Vet Med Sci. 2016;7:130-137.https://doi.org/10.36326/kjvs/2016/v7i24340
  13. Ihasn FR. Low-level laser therapy accelerates collateral circulation and enhances microcirculation. J Photomed Laser Surg. 2005;23:289-294.https://doi.org/10.1089/pho.2005.23.289
  14. Gal P, Vidinsky B, Toporcer T, Mokry M, Mozes T, Longauer F, et al. Histological assessment of the effect of laser irradiation on skin wound healing in rats. Photomed Laser Surg. 2006;24:480-488.https://doi.org/10.1089/pho.2006.24.480
  15. Bjordal JM, Johanson ML, Iversen V, Aimbire F, Lopes-martins RA. Low-level laser therapy in acute pain: a systematic review of possible mechanisms of action and clinical effects in randomized placebo-controlled trials. J Photomed Laser Surg. 2006;24:158-167.https://doi.org/10.1089/pho.2006.24.158
  16. Araujo CE, Ribeiro S, Favaro R, Zezell DM, Zorn TM. Ultra-structural and autoradiographical analysis show a faster skin repair in HeNe laser-treated wound. J Photochem Photobiol B. 2007;86:87-96.https://doi.org/10.1016/j.jphotobiol.2006.08.006
  17. Nguyen DT, Orgill DP, Murphy GT. The pathophysiologic basis for wound healing and cutaneous regeneration. In: Orgill DP, Blanco C, editors. Biomaterials for Treating Skin Loss. Elsevier; 2009. p. 25-57.https://doi.org/10.1533/9781845695545.1.25
  18. Thangapazham RL, Sharma A, Maheshwari RK. Beneficial role of curcumin in skin diseases. Adv Exp Med Biol. 2007;595:343-357.https://doi.org/10.1007/978-0-387-46401-5_15
  19. Gong C, Wu Q, Wang Y, Zhang D, Luo F, Zhao X, et al. A biodegradable hydrogel system containing curcumin encapsulated in micelles for cutaneous wound healing. Biomaterials. 2013;34:6377-6387.https://doi.org/10.1016/j.biomaterials.2013.05.005
  20. Campos AC, Groth AK, Branco AB. Assessment and nutritional aspects of wound healing. Curr Opin Clin Nutr Metab Care. 2008;11(3):281-284.https://doi.org/10.1097/MCO.0b013e3282fbd35a
  21. Urosevic M, Nikolic L, Gajic I, Nikolic V, Dinic A, Miljkovic V. Curcumin: Biological activities and modern pharmaceutical forms. Antibiotics (Basel). 2022;11(2):135.https://doi.org/10.3390/antibiotics11020135
  22. Hawkins D, Abrahamse H. Effect of multiple exposure of low-level laser therapy on the cellular responses of wounded human skin fibroblasts. J Photomed Laser Surg. 2006;24:705-714.https://doi.org/10.1089/pho.2006.24.705
  23. Patil SS, Bhasarkar S, Rathod VK. Extraction of curcuminoids from Curcuma longa: comparative study between batch extraction and novel three-phase partitioning. Prep Biochem Biotechnol. 2019;49(4):407-418.https://doi.org/10.1080/10826068.2019.1575859
  24. Chhetri HP, Yogol NS, Sherchan J, Anupa KC, Mansoor S, Thapa P. Formulation and evaluation of antimicrobial herbal ointment. Kathmandu Univ J Sci Eng Technol. 2010;6(1):102-107.https://doi.org/10.3126/kuset.v6i1.3317
  25. Sharma S, Singh SP. Dermatological preparations, formulation, and evaluation of various semi-solid dosage forms. Asian J Pharm Res Dev. 2014;10-25.
  26. Marriott JF, Wilson KA, Langley CA, Belcher D. Pharmaceutical Compounding and Dispensing. 2nd ed. Pharmaceutical Press; 2010. p. 163.
  27. Lane ME. Ointments, pastes, gels, cutaneous patches, and topical sprays. In: Taylor KM, Aulton ME, editors. Aulton's Pharmaceutics E-Book: The Design and Manufacture of Medicines. 6th ed. Elsevier Health Sciences; 2022. p. 453-462.
  28. d'Acampora AJ, Vieira DS, Silva MT, Farias DC, Tramonte R. Morphological analysis of three wound-cleaning processes on potentially contaminated wounds in rats. Acta Cir Bras. 2006;21:332-340.https://doi.org/10.1590/S0102-86502006000500011
  29. Sardari K, Dehgan MM, Mohri M, Emami MR, Mirshahi A, Maleki M, et al. Macroscopic aspects of wound healing (contraction and epithelialization) after topical administration of allicin in dogs. Comp Clin Pathol. 2006;15(4):231-235.https://doi.org/10.1007/s00580-006-0634-2
  30. Patil MV, Kandhare AD, Bhise SD. Pharmacological evaluation of ethanolic extract of Daucus carota Linn root formulated cream on wound healing using excision and incision wound model. Asian Pac J Trop Biomed. 2012;2(2):S646-S655.https://doi.org/10.1016/S2221-1691(12)60290-1
  31. Weinberg SL, Abramowitz SK. Statistics using SPSS: An integrative approach. Cambridge University Press; 2008.
  32. Luna IG. Manual of Histology Staining Methods of the Armed Forces Institute & Pathology. 3rd ed. McGraw-Hill; 1968. p. 34-215.
  33. Estevao LD, Vieira P, Leite AGB, Bulhoes AV, Barcelos LS, Neto J. Morphological evaluation of wound healing events in the excisional wound healing model in rats. Bio-protocol. 2019;9(13):e3285.https://doi.org/10.21769/BioProtoc.3285
  34. Gal P, Kilik R, Mokry M, et al. Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: standardization of semi-quantitative and quantitative histological assessments. Vet Med (Praha). 2008;53:652-659.https://doi.org/10.17221/1973-VETMED
  35. Dancáková L, Vasilenko T, Kováč I, Jakubčová K, Hollý M, Revajová V, et al. Low-level laser therapy with 810 nm wavelength improves skin wound healing in rats with streptozotocin-induced diabetes. Photomed Laser Surg. 2014;32(4):198-204.https://doi.org/10.1089/pho.2013.3586
  36. Gupta A, Kumar P. Assessment of the histological state of the healing wound. Plast Aesthet Res. 2015;2:239-242.https://doi.org/10.4103/2347-9264.158862
  37. Cañedo-Dorantes L, Cañedo-Ayala M. Skin acute wound healing: a comprehensive review. Int J Inflamm. 2019;2019:1-15.https://doi.org/10.1155/2019/3706315
  38. Wilkinson HN, Hardman MJ. Wound healing: Cellular mechanisms and pathological outcomes. Open Biol. 2020;10(9):200223.https://doi.org/10.1098/rsob.200223
  39. Topaloglu N, Güney M, Yuksel S, Gülsoy M. Antibacterial photodynamic therapy with 808-nm laser and indocyanine green on abrasion wound models. J Biomed Opt. 2015;20(2):028003.https://doi.org/10.1117/1.JBO.20.2.028003
  40. Atiyeh BS, Ioannovich J, Al-Amm CA, El-Musa KA. Management of acute and chronic open wounds: the importance of a moist environment in optimal wound healing. Curr Pharm Biotechnol. 2002;3(3):179-195.https://doi.org/10.2174/1389201023378283
  41. Medrado AP, Soares AP, Santos ET, Reis SRA, Andrade ZA. Influence of laser photobiomodulation upon connective tissue remodeling during wound healing. J Photochem Photobiol B. 2008;92(3):144-152.https://doi.org/10.1016/j.jphotobiol.2008.05.008
  42. Wardlaw JL, Gazzola KM, Wagoner A, Brinkman E, Burt J, Butler R, et al. Laser therapy for incision healing in 9 dogs. Front Vet Sci. 2019;5:349.https://doi.org/10.3389/fvets.2018.00349
  43. Mani H, Sidhu GS, Kumari R, Gaddipati JP, Seth P, Maheshwari RK. Curcumin differentially regulates TGF-β1, its receptors, and nitric oxide synthase during impaired wound healing. Biofactors. 2002;16(1-2):29-43.https://doi.org/10.1002/biof.5520160104
  44. Akbik D, Ghadiri M, Chrzanowski W, Rohanizadeh R. Curcumin as a wound healing agent. Life Sci. 2014;116(1):1-7.https://doi.org/10.1016/j.lfs.2014.08.016
  45. Asouri M, Ataee R, Ahmadi AA, Amini A, Moshaei MR. Antioxidant and free radical scavenging activities of curcumin. Asian J Chem. 2013;25(13):7593-7595.https://doi.org/10.14233/ajchem.2013.15308
  46. Kulac M, Aktas C, Tulubas F, Uygur R, Kanter M, Erboga M, et al. The effects of topical treatment with curcumin on burn wound healing in rats. J Mol Histol. 2013;44(1):83-90.
  47. https://doi.org/10.1007/s10735-012-9452-9
  48. Kant V, Gopal A, Pathak NN, Kumar P, Tandan SK, Kumar D. Antioxidant and anti-inflammatory potential of curcumin accelerated the cutaneous wound healing in streptozotocin-induced diabetic rats. Int Immunopharmacol. 2014;20(2):322-330.https://doi.org/10.1016/j.intimp.2014.03.009
  49. Prasad R, Kumar D, Kant V, Tandan SK, Kumar D. Curcumin enhanced cutaneous wound healing by modulating cytokines and transforming growth factor in excision wound model in rats. Int J Curr Microbiol Appl Sci. 2017;6:2263-2273.https://doi.org/10.20546/ijcmas.2017.607.266
  50. Panchatcharam M, Miriyala S, Gayathri VS, Suguna L. Curcumin improves wound healing by modulating collagen and decreasing reactive oxygen species. Mol Cell Biochem. 2006;290(1-2):87-96.https://doi.org/10.1007/s11010-006-9170-2
Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 21, Issue 2
June 2022
Pages 116-127
Files
Share
How to cite
Statistics