Document Type : Al-Qadisiyah Conference 2025
Authors
1 جامعة كربلاء / كلية العلوم الطبية التطبيقية
2 Department of Microbiology and Parasitology, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq.
Abstract
This study was acted in Karbala and Qadisiyah governarat center of Iraq in perioed from Septemper2021 to October 2022 request to location of the crafty Free living amoebae (FLA) and recognized by morphological characters in culture, Polymeras chain response (PCR) by 18SrRNA general FLA primers. 181 samples were gathered from 123 clinical cases including, eye, skin, nose from animals and human, and CSF from human and 58 samples were gathered from environmental samples were gathered from various sources of water , soil .All samples were refined in the Non Supplement agar medium (NN-agar medina) and then inspected by light microscope to perceived Morphologically the flagellate, trophozoite and cyst of opportunistic amoebas for 12/181 samples included 5/58(2.276%) environmental source (water and soil ) and 7/123( 3.867%) clinical(eye,skin,nose samples) from only animals were positive to Naegleria spp. Ten from12 tests from environmental and clinical sources were disconnected of common FLA that were positive microscopically, the outcome was positive for three samples for Naegleria gruberi after analyzed sequences. The study of isolation and molecular diagnosis of Naegleria gruberi is the first of its kind in Iraq, and It is the first recorded in Iraq in the Gene Bank database.
Keywords
)