Author
College of Biotechnology/ University of AL-Qadisiyah , Iraq
Abstract
The principles of this work were to isolate the bacterium Salmonella and evaluate its antibiotic resistance capability from rectal fecal samples of some types of animals from Al-Diwaniyah City, Iraq. A total of 38 randomly collected samples (13, 16, and 9) from healthy cows, sheep, and goats, respectively, were employed to take samples via the use of commercially-available sterile cotton swabs. The samples were cultivated using different agar media. Then, the suspected Salmonella growth from the nutrient agar was subjected to biochemical identification. Later, antibiotic susceptibility assay was performed on the pure Salmonella isolates using Mueller-Hinton (M-H) agar and via the use of Kirby-Bauer (K-B) method. The cultivation and biochemical tests resulted in 7 (53%) for cows, 5 (31%) for sheep, and 5 (55.5%) for goats) pure isolates of Salmonella spp. Antibiotic resistance was varied among the isolates with MAR index of 0.39 for cows, 0.32 for sheep, and 0.24 for goats. Most isolates were resistant to almost all antibiotics, especially streptomycin, ampicillin, kanamycin, and novobiocin. The current findings reveal that fecal samples from cows, sheep, and goats have Salmonella spp with antibiotic resistance traits.
Keywords
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